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. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilliermond - Atkinson — 198 — Cytoplasm Relationships between the Golgi apparatus and the chondrio- somes and plastids:- The above results have been a subject of much debate in animal cytology, and various authors, among others BowEN and Gatenby, Duboscq and Grasse have not been able to confirm the observations of A. CoRTi and Parat. It must be noticed that all these authors abandoned silver methods and used only osmic methods. There was therefore the question as to whether osmic methods produce the same results as the s
. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilliermond - Atkinson — 198 — Cytoplasm Relationships between the Golgi apparatus and the chondrio- somes and plastids:- The above results have been a subject of much debate in animal cytology, and various authors, among others BowEN and Gatenby, Duboscq and Grasse have not been able to confirm the observations of A. CoRTi and Parat. It must be noticed that all these authors abandoned silver methods and used only osmic methods. There was therefore the question as to whether osmic methods produce the same results as the silver methods. Work which we have done using these methods on plant cells has shown us that the osmic methods are much less specific than the silver methods. If the impregnations have lasted only a week, there is a blackening of the vacuolar system only when it encloses. Fig. 135. — Vicia Faba. Cells in the seed before maturation. Silver impregnation of da Fano. 1-6, parenchymatous cells of the cotyledon; 1-4, Golgi net- work. 5-6, deprived of oxygen, the Golgi network breaks up into vacuoles containing silver-impregnated precipitates. 7, epidermal cells of the integument of * the seed; Golgi network. (After Sanchez). tannins, which instantaneously reduce osmic acid. Otherwise, it is the chondriome which is impregnated and it may be well pre- served but is often vesiculated. If the impregnation is prolonged to two weeks, there is a more profound alteration of the plastids and chondriosomes, which become large vesicles and sometimes even anastomose into a fine network, much like the network of Golgi, but now the vacuolar system may also be impregnated. These impregnations are then very irregular and it is not rare to ob- serve, side by side in the same section, cells in which the chondri- ome alone is blackened, sometimes well-preserved, sometimes strongly vesiculated, and other cells in which the chondriome and the vacuolar system are both blackened, still other cells in
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Keywords: ., bookcentury1900, bookcollection, booksubjectplantcellsandtissues