. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 114 U. ABDU ET AL. -I 3,00- cis-trans MF Retention time (min ) Figure 1. HPLC detection of methyl farnesoate in Macrdbnicliiuin rosenbergii larvae at stage 4. Cis-trans methyl farnesoate was used as the internal standard. pleopods and the walking legs are not in the same plane, whereas in post-larvae, the abdomen is straight, with the pleopods and the walking legs in the same plane. (2) Larvae swim mainly by beating the long exopodites of the pereiopods. During the metamorphic process these lon
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 114 U. ABDU ET AL. -I 3,00- cis-trans MF Retention time (min ) Figure 1. HPLC detection of methyl farnesoate in Macrdbnicliiuin rosenbergii larvae at stage 4. Cis-trans methyl farnesoate was used as the internal standard. pleopods and the walking legs are not in the same plane, whereas in post-larvae, the abdomen is straight, with the pleopods and the walking legs in the same plane. (2) Larvae swim mainly by beating the long exopodites of the pereiopods. During the metamorphic process these long exopodites degenerate and become reduced to knobs at the postmetamorphic molt (Ling, 1969). (3) In stage 11 larvae, the endopodite of the second pereiopod has three segments, the second and third segments being at an angle to the first, which is not fully developed. In contrast, in post-larvae there are four segments, all of which are straight, and the first segment is fully devel- oped, as in the adult. (4) The carapace median tooth is evident from larval stage 4 onward, but disappears during metamorphosis and is absent in the post-larva. (5) In stage I 1 larvae, the entire dorsal margin of the rostrum is equipped with numerous small teeth, but no ventral teeth are present, whereas the rostrum of the post-larva has I1 large dorsal teeth and 5 ventral teeth. (6) Larvae are planktonic. swimming tail up. and in most cases tail first, ventral side up; during metamorphosis, they settle to the bottom. Post-larvae are benthic, swimming and behaving as do the juveniles. The relative abundance of the different intermediate types were compared between treatments, and the statisti- cal significance was tested using the Mann-Whitney U test. Results Presence of methyl farnesoate in Macrobrachium rosenbergii larvae MF was found in hexane extracts of M. rosenbergii larvae at stages 4, 6, 9, 10, and 11, and in post-larvae. A 50,000 1 <u U c 03 3 -C. 25,000 - 69 n"1 40 lUillU 114 ili
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