. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ! s (.11 \( Kl \Hl Ml \\I) II Kl I I I ^ Kigure 3. Time course of incorporation of IJ(. leucine mlo proteins from muscle (triangles), ovaries (circles), and hepatopancreas (squares) from either intact crabs (open s\ m hols I or evestalk-ablated crahs (tilled v. mhnls) measured in \iir Ml values are means ± one standard error. n = 12 for each group. 2 replicates ture of protein svnthesis. A 4-h incubation time was used for all subsequent assays. Crude shrimp eyestalk extracts \\crc tested for their ability to atl'ect /';; vit
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ! s (.11 \( Kl \Hl Ml \\I) II Kl I I I ^ Kigure 3. Time course of incorporation of IJ(. leucine mlo proteins from muscle (triangles), ovaries (circles), and hepatopancreas (squares) from either intact crabs (open s\ m hols I or evestalk-ablated crahs (tilled v. mhnls) measured in \iir Ml values are means ± one standard error. n = 12 for each group. 2 replicates ture of protein svnthesis. A 4-h incubation time was used for all subsequent assays. Crude shrimp eyestalk extracts \\crc tested for their ability to atl'ect /';; vitro protein synthesis in the ovaries from intact and eyestalk-ablated crabs (Fig. 4). The threshold tor statistically significant inhibition of protein svnthesis uas the same regardless of the origin of the ovarian tissues ( FSI . /-test for two percentages. /' OOH Maximum inhibition of the protein synthesis was produced b> FSI in ovaries from eyestalk ab- crabs. l'i it n sv nthcsis in ovaries from intact crabs was maximal: ii , bited by ESE. Ovaries from the intact crabs were more sensitive to the evcstalk extract than ovaries I: .] crabs. \ dose of FSF produced a sifmlican .iler inhibition in the ovaries from intact crabs than , d in the ovaries from evestalk ablated crabs (/-test for i ''- o.(>5). I he highest dose tested. I \i ;.,nduccd less, not more, inhibition of protein synthesis a lower dose I Ins suggests that the crude extract probably contains many factors, perhaps even a factor that can increase protein synthesis /// w'Zro (Charniaux-Cotton, 1 1>S5). I 'itellogenin intrittcation and antibody characterization The partially purified extract of ovaries from lator was dominated by the two distinct bands of protein characteristic of the egg yolk proteins (Eastman-Reks and Fingerman. 1984: Fig. 5A. B). The molecular weights of the two groups of egg yolk proteins were 1 ± lOOOdaltons. V,. and 8 1,000 ± lOO
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
