. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 2. Skate erythrocyte cytoskeletons (Triton X-100 lysis), as observed in phase contrast under oil immersion. Centrioles appear as paired, phase-dense "dots" (arrowheads), (a) One of the centrioles very close to or in contact with MB; N = nucleus, (b) Centriole pair adjacent to, but not in direct contact with major part of MB; (c) centriole pair appearing to touch MB, with a "fiber" (f) extending away from it. some of the centriole pairs are found to be attached to fibers (microtubule bundles) which
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 2. Skate erythrocyte cytoskeletons (Triton X-100 lysis), as observed in phase contrast under oil immersion. Centrioles appear as paired, phase-dense "dots" (arrowheads), (a) One of the centrioles very close to or in contact with MB; N = nucleus, (b) Centriole pair adjacent to, but not in direct contact with major part of MB; (c) centriole pair appearing to touch MB, with a "fiber" (f) extending away from it. some of the centriole pairs are found to be attached to fibers (microtubule bundles) which are part of the MB, or which extend from the centrioles toward a distant point on the MB (Fig. 3). Confirmation of the attachment of centrioles to the MB, including some which appeared separated from the main body of the MB, was obtained by examination of cytoskeleton whole mounts in TEM. In Figure 4 the centrioles are observed to be attached to only one or possibly a few microtubules of the spread MB. Figure 5 shows centrioles which, in phase contrast, would appear to be close to, but not touching the MB, but which are actually connected to it by radiating microtubules. Examination of the centrioles in underexposed prints revealed their cylindrical shape, microtubu- lar substructure, and sometimes (as in Fig. 5) their approximately orthogonal orienta-. FIGURE 3. Cytoskeletons flattened under the coverslip to provide improved higher magnification views of centriole-associated fibers in phase contrast. Such fibers appear to be part of the MB (a, b; arrow- heads), or to extend from at least one of the centrioles toward a distant point on the MB (c; arrowhead). Flattening also generally produced an artifactual increase in area of nucleus, as in Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biologic
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
