. Carnegie Institution of Washington publication. BROWN ROT OF SOLANACEAE. I83 Result.—Successful; the first signs appeared on the fifth day; weather exceedingly hot for last 4 days. The diseased stems of potato were swarming with short motile rods. Illustrated in Bull. 12, Division of Vegetable Phy- siology and Pathology, 1896, pi. 1, fig. 1. Tune S.—Fifteen inoculations were made on pear tree (Japan), pepper, pepino, Solanum nigrum, Datura stramonium, tobacco, tomato, potato, using slant agar No. 1 of June 4, from culture used for inoculations of May 27. Each plant received many pricks. Resu
. Carnegie Institution of Washington publication. BROWN ROT OF SOLANACEAE. I83 Result.—Successful; the first signs appeared on the fifth day; weather exceedingly hot for last 4 days. The diseased stems of potato were swarming with short motile rods. Illustrated in Bull. 12, Division of Vegetable Phy- siology and Pathology, 1896, pi. 1, fig. 1. Tune S.—Fifteen inoculations were made on pear tree (Japan), pepper, pepino, Solanum nigrum, Datura stramonium, tobacco, tomato, potato, using slant agar No. 1 of June 4, from culture used for inoculations of May 27. Each plant received many pricks. Result.—Succeeded on tomato, potato, Solanum nigrum, and Datura stramonium (fig. 88). The organisms in the diseased Daturas were short, motile bacteria. June 15.—Sixteen inoculations into Pelargonium zonale. Datura stramonium, Solatium carolinense, potato and tomato, were made with cultures derived from the same source as those used for the inoculations of June 8 and May 27. Result.—Failed, except on potato, the stem and tubers of which finally rotted. There was only a local multiplication of the bacteria in 2 green fruits of the tomato. June 21.—The plants used for these inoculations were 40 strong, growing tomato vines 10 inches high in 4-inch pots. Nos. 46 to 66 were pricked in middle of stem, and Nos. 67 to 85 in the tender tip of stem; many deep pricks were made in each case. The culture used was slant agar No. 3 of June iS, whose history is as follows: Made from a single colony on poured plate No. 3, June 14, from slant agar No. 1, June n, which was made directly from interior of stem of potato No. 13, inoculated May 27. Result.—Failed; at least there were no signs for 30 days. Possibly a wrong colony was used; at any rate the method was defective, as plate cultures should have been made direct from plant No. 13. July 5.—Egg-plants, cucumbers, tobacco, potato, Datura stramonium were used for these inoculations (30 in all). The leaves and stems were pricked. Th
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