A text-book of physiology for medical students and physicians . aBC Eb Fig. —Diagram to show the variations in theabsorption spectrum of oxyhemoglobin with varyingconcentrations of the solution.—(After Rolleit.) Thenumbers to the right give the strength of the oxy-hemoglobin solution in percentages; the letters givethe positions of the Fraunhofer lines. To ascertainthe amount of absorption for any given concentrationup to 1 per cent., draw a horizontal line across thediagram at the level corresponding to the concentra-tion. Where this line passes through the shaded partof the diagram abso
A text-book of physiology for medical students and physicians . aBC Eb Fig. —Diagram to show the variations in theabsorption spectrum of oxyhemoglobin with varyingconcentrations of the solution.—(After Rolleit.) Thenumbers to the right give the strength of the oxy-hemoglobin solution in percentages; the letters givethe positions of the Fraunhofer lines. To ascertainthe amount of absorption for any given concentrationup to 1 per cent., draw a horizontal line across thediagram at the level corresponding to the concentra-tion. Where this line passes through the shaded partof the diagram absorption takes place, and the widthof the absorption bands is seen at once. The diagramshows clearly that the amount of absorption increasesas the solutions become more concentrated, especiallythe absorption of the blue end of the spectrum. Itwill be noticed that with concentrations between per cent, the two bands between D and E fuseinto one. GENERAL PROPERTIES: THE CORPUSCLES. 427. aBC Eb trum with two absorption bands closely resembling in posi-tion and appearance those of oxyhemoglobin. They are dis-tinguished from theoxyhemoglobin bandsby being slightlynearer the blue endof the spectrum, asmay be demonstratedby observing the wavelengths or, more con-veniently, by super-posing the two , solutions ofcarbon monoxid hem-oglobin are not re-duced to hemoglobinby adding Stokessliquid, two bands be-ing still seen after suchtreatment. A solu-tion of carbon mon-oxid hemoglobin suit-able for spectroscopicexamination may beprepared easily bypassing ordinary coal-gas through a dilute oxyhemoglobin solution for a few minutesand then filtering. Derivative Compounds of Hemoglobin.—There are a numberof pigmentary bodies which are formed directly from hemoglobinby decompositions or chemical reactions of various kinds. Someof these derivative substances occur normally in the body. Thebest known are as follows *: Methemoglobin.—When blood or a solution of oxy
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