. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 168 ALFRED M. ELLIOTT AND ROBERT L. HUNTER separated by centrifugation. Appropriate dilutions of the supernatant were made with N/20 NaOH and absorption determinations The results of this study indicated that the adenine level remained constant throughout the period of nucleo- tide breakdown. Apparently, then, the enzyme action takes place either within the cell or at the membrane and perhaps the value of this reaction is to obtain energy for cellular metabolism. Relation to pH The activity of the enzymes in relation
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 168 ALFRED M. ELLIOTT AND ROBERT L. HUNTER separated by centrifugation. Appropriate dilutions of the supernatant were made with N/20 NaOH and absorption determinations The results of this study indicated that the adenine level remained constant throughout the period of nucleo- tide breakdown. Apparently, then, the enzyme action takes place either within the cell or at the membrane and perhaps the value of this reaction is to obtain energy for cellular metabolism. Relation to pH The activity of the enzymes in relation to pH was demonstrated by following the inorganic phosphate release from adenylic acid at various pH levels. The re- sults are recorded in Figure 3 where the organisms released the greatest amount /ml. 15 10. P-D pH FIGURE 3. Phosphate release at various pH levels. of phosphate at two levels, namely, and Since the system is complicated by intact cells in the medium, it is impossible to say that the nucleotidase activity is influenced by pH in the manner shown by these experiments, although it may be. It might seem strange that all of the experiments were maintained at pH rather than at the levels where enzyme activity in this system was optimal. It so happens that both of these levels are outside the range for optimal growth, and therefore might be injurious to the organism. There seems to be no correlation between pH levels which promote optimal phosphatase activity in living cells and those that support optimal growth of those cells. 3 The adenine determinations were made through the courtesy of Dr. James E. Hogg, Department of Biological Chemistry, University of Michigan. The absorptions of adenine, adenylic acid and adenosine do not differ more than 3 per cent in N/2Q NaOH solution (Morton, 1942).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearan
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