. Principles of agricultural chemistry [microform] . Fig- 53-—Peas without nitrogen (KP) grow as well as with nitrogen(KPS) but oats do not. Wagner. CHEMICAI. CHANGES 227 Further, the plant absorbs the bacteria, and they disappear.(3) Nobbe and Hiltner found that the stronger the plant, thegreater the resistance it offers to inoculation, and the sooner thenodules are emptied. Exactly how the nitrogen is transferred from bacteria to plantis not known. Inoculation of Legumes.—When a legume is planted in newlocalities, the bacteria suitable to it may not be present to aid it. Fig. 54.—Showing dif
. Principles of agricultural chemistry [microform] . Fig- 53-—Peas without nitrogen (KP) grow as well as with nitrogen(KPS) but oats do not. Wagner. CHEMICAI. CHANGES 227 Further, the plant absorbs the bacteria, and they disappear.(3) Nobbe and Hiltner found that the stronger the plant, thegreater the resistance it offers to inoculation, and the sooner thenodules are emptied. Exactly how the nitrogen is transferred from bacteria to plantis not known. Inoculation of Legumes.—When a legume is planted in newlocalities, the bacteria suitable to it may not be present to aid it. Fig. 54.—Showing difference in the growth of alfalfa caused byinoculation with bacteria. Illinois Station. in the assimilation of nitrogen. The safest plan is to inoculatethe soil with the proper bacteria. There are two methods of doingthis. (i) The first method consists in inoculating with soil from afield where the plants have been growing well. This is the surestmethod, but open to some objections. Freight charges on thesoil may be expensive; the soil may be difficult to secure; injuriousinsects or diseases or weeds are likely to be brought into the soil. 228 PRINCIPLES OF AGRICULTURAL CHEMISTRY (2) The second method^ consists in inoculating the soil or seedwith a pure culture of the necessary organisms. This methodwas patented in Germany in 1880, but did not prove a commercialsuccess. The method has been modified, and cultures arenow upon the market. Pure cultures of the symbioticbacteria may easily be prepared by inoculating a suitablesterilized culture medium with the bacteria
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