. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 450 C. M. Nil AND L. MUSCATINE 00 I symbiotic. 0 uc D symbiotic. 300 nE symbiotic, 300iiE. DCMU. 26 32 temperature (°C) Figure 5. Superoxide production by symbiotic Aiptaxia pulchella at ambient (26"C) and elevated (32°C) temperature in darkness, in light, and in light in the presence of mM DCMU. Groups of six anemones were used in each treatment. A 60-min NET reduction experiment was then conducted under each defined condition, after which the amount of formazan produced by each anemone was measured. Each bar rep-
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 450 C. M. Nil AND L. MUSCATINE 00 I symbiotic. 0 uc D symbiotic. 300 nE symbiotic, 300iiE. DCMU. 26 32 temperature (°C) Figure 5. Superoxide production by symbiotic Aiptaxia pulchella at ambient (26"C) and elevated (32°C) temperature in darkness, in light, and in light in the presence of mM DCMU. Groups of six anemones were used in each treatment. A 60-min NET reduction experiment was then conducted under each defined condition, after which the amount of formazan produced by each anemone was measured. Each bar rep- resents the mean ± SD for six anemones per treatment. stress to occur in these anemones, aposymbiotic A. pul- chella were exposed to FSW containing 1CT4 M NBT in both darkness and light at both ambient and elevated temperature. Figure 6 shows that temperature had a sig- nificant effect on formazan production by intact apo- symbiotic anemones (ANOVA, P < ): in darkness and light these anemones produced significantly more formazan at elevated temperature (Kruskal-Wallis, P and P > , respectively). Interestingly, at ambient temperature, formazan production in darkness was sig- nificantly higher by aposymbiotic anemones than by symbiotic anemones in darkness (Kruskal-Wallis, P 5 nmol; Auclair and Voisin, 1985). How- ever, torn: > was not detected in the external medium under any >i he incubation conditions (data not shown). If these algae released superoxide ions under these exper- imental conditions, the amount was less than 5 nmol. SOD and CAT activity in symbiotic and aposymbiotic A. pulchella To determine if differences in NBT reduction were re- lated to the ability of the host to detoxify potential oxi- dants, the specific activities of Cu-Zn SOD, Mn SOD, and CAT were measured in animal homogenates pre- pared from symbiotic and aposymbiotic A. pulchella. The specific activities of Cu-Zn SOD (Mann-Whitney U; P < , Fig. 7), Mn SOD (Mann-Wh
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