. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 70 S. ZIELINSKI ET AL analysis of cooperativity at a specific pH. If this is done in the range of saturation S between and , the analysis leads to an estimate of Hill coefficients (H5()) according to log(S/l - S) = H5(,(log Po: - log P50) (3) where Po-, is the Po2 of the isobar, S results from Pen at a specific pH (pH50), and P50 is the Po, for S = at the same pH (Former, 1990). Results The concentration of hemocyanin in native blood (he- molymph) was 93 g 1~' for Megaleledone senoi and 142 g 1 ' for Sepia officin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 70 S. ZIELINSKI ET AL analysis of cooperativity at a specific pH. If this is done in the range of saturation S between and , the analysis leads to an estimate of Hill coefficients (H5()) according to log(S/l - S) = H5(,(log Po: - log P50) (3) where Po-, is the Po2 of the isobar, S results from Pen at a specific pH (pH50), and P50 is the Po, for S = at the same pH (Former, 1990). Results The concentration of hemocyanin in native blood (he- molymph) was 93 g 1~' for Megaleledone senoi and 142 g 1 ' for Sepia officinalis. This is equivalent to a maximum level of hemocyanin-bound oxygen of mmol O: 1~' in the octopod and of mmol 1~' in Sepia. For M. senoi hemocyanin, the highest pH sensi- tivity of oxygen binding was found at 10 °C, as indicated by maximum slopes AS/ApH (Fig. 2). Lower tempera- tures resulted in a somewhat decreased pH sensitivity of oxygen affinity, with a maximum of AS/ApH = 13% per pH unit at 10 °C, compared to a maximum of 10% per pH unit at 0 °C. Saturation at 0 °C did not fall below 43% even at low pH ( and ) and low Po2 (1 kPa). At 10 °C, saturation dropped to a minimum of 32% at the same 100 r — 50% S-. PH Figure 2. Continuous analysis of the relationships between oxygen binding, pH. and Po, in whole blood of the Antarctic octopod Megaleledone senoi at temperatures between 0 and 10 °C (S = saturation; points were chosen at regular intervals from the continuous recordings).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clar
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
