Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . Method of Schlbsing. This method is the one most commonly used, but is open to the objectionthat it does not yield accurate results and is time-consuming, but has theadvantage of simplicity. It is argued by many that the element of time is ofno importance, as clinically one would not wait for an ammonia determination 2l6 DIAGNOSTIC METHODS. before instituting vigorous treatment. On the other hand, in metabolic workit is of a great advantage to get the work out of the way as quickly as
Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . Method of Schlbsing. This method is the one most commonly used, but is open to the objectionthat it does not yield accurate results and is time-consuming, but has theadvantage of simplicity. It is argued by many that the element of time is ofno importance, as clinically one would not wait for an ammonia determination 2l6 DIAGNOSTIC METHODS. before instituting vigorous treatment. On the other hand, in metabolic workit is of a great advantage to get the work out of the way as quickly as is possibleand consistent with accurate results. Technic. Twenty-five of urine are placed in the vessel B (preferably a Petri dish)(see cut). Above this is placed a glass triangle upon which rests a dish (C)containing 20 of tenth-normal sulphuric acid. Twenty of milk oflime are then poured into the dish containing the urine and the whole coveredwith a bell-jar, the borders of which have been well greased to make an air-tight union when the jar is placed upon the glass plate. This apparatus. Fig. 75.—Schlosings ammonia apparatus. is then allowed to stand at room temperature from four to five days, duringwhich time the ammonia, liberated by the action of the milk of lime uponthe, ammonium salts of the urine, will be taken up by the sulphuric acid inthe vessel C. At the end of this time the bell-jar is removed, the acid titratedwith tenth-normal sodium hydrate, and the number of of remaining aciddetermined. One of tenth-normal sulphuric acid neutralized by theevolved ammonia represents gram of ammonia. This figure ismultiplied by 4 to obtain the percentage ammonia value. If any moisture ispresent on the inside of the bell-jar it should be washed into the sulphuricacid before titration. This method, as previously stated, does not give accurate results • owingto the fact that ammonia may be split off from urea and thus give figureswhich are som
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