The Journal of biological chemistry . pection of Equation 14 it is evident that the fraction . 7^^—:—,-rj, .? expressing the effects of the cell factors, is 2 (B)c — (BP)c + (Hb) at a given pH, constant for all bloods, whether of high or lowhemoglobin content, as long as the ratio of base to hemoglobin inthe cells remains constant. For then all the terms in both numer-ator and denominator vary directly as (Hb) (see Equations 15 (BP).and 55). The second fraction of Equation 14, c,^. ZTTrfTT expressing the effect of the serum factors, varies slightly, atconstant pH, with the hemoglobin content o
The Journal of biological chemistry . pection of Equation 14 it is evident that the fraction . 7^^—:—,-rj, .? expressing the effects of the cell factors, is 2 (B)c — (BP)c + (Hb) at a given pH, constant for all bloods, whether of high or lowhemoglobin content, as long as the ratio of base to hemoglobin inthe cells remains constant. For then all the terms in both numer-ator and denominator vary directly as (Hb) (see Equations 15 (BP).and 55). The second fraction of Equation 14, c,^. ZTTrfTT expressing the effect of the serum factors, varies slightly, atconstant pH, with the hemoglobin content of the blood. Butthe variation is so small, and the total effect of this fraction on the 778 Electrolyte and Water Distribution value of r relatively so little, that the r value is, within the limitsof experimental determination, independent of the hemoglobincontent of the blood, even when the latter varies over such a widerange as from 3 to 12 millimolar, corresponding to from 7 to 27cc. of oxygen capacity per 100 gm. of Fig. 1. Values of r = [H+]. _ [Cll, [HCOslc [H+]. [Cl]s [HCO3].calculated by Equation 14 for horse blood of average serum and cell compo-sition observed in the four experiments reported in this paper are indicatedby the curves. The observed chloride and bicarbonate ratios in ourexperiments are indicated by the marked points. Consequently we may represent the average normal r, pHrelationship by a single curve, which holds for bloods of varyinghemoglobin content, if the other non-diffusible constituentsmaintain towards the hemoglobin the relationships indicated byEquations 15, 16, and 17. The curves obtained by substituting inEquation 14 the values for (B),, and (B)s indicated by Equa- Van Slyke, Wu, and McLean 779 tions 15 and 16, (when Hb = 9) and the values for (BP)«calculatedfrom the (P)s indicated by Equation 17, is given in Fig. 1. Inplotting the curves the values of r were obtained by solvingEquation 14 by a method of repeated approximation,
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