Archive image from page 34 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 were detected as positive for the marker, whether determined by PCR or hybridization, were next digested with restriction endonucleases and the resulting fragments electrophoresed on an agarose gel. The gel allows a determination of the overlap between cosmids for a particular marker and allows the production of a cosmid map for the marker. A Southern Blot is used to determine the exact fragment of the restriction endonuclease digestion containing
Archive image from page 34 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 were detected as positive for the marker, whether determined by PCR or hybridization, were next digested with restriction endonucleases and the resulting fragments electrophoresed on an agarose gel. The gel allows a determination of the overlap between cosmids for a particular marker and allows the production of a cosmid map for the marker. A Southern Blot is used to determine the exact fragment of the restriction endonuclease digestion containing the marker sequence. Restriction Endonuclease Digestion and Agarose Gel Electrophoresis
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