. Biological structure and function; proceedings. Biochemistry; Cytology. 58 F. EDMUND HUNTER, JR. just as they do other swelhng agents. Possibly they should be grouped with EDTA. As has been observed in other work Mn + + is considerably more effective than Mg + + in preserving mitochondrial structure. Because of 20 30 40 MINUTES Fig. 6. Inhibition of ascorbate-induced lysis of mitochondria by metal com- plexing agents. o CO ID < - - -â¢ââ¢â â¢â⢠« â¢v^ ASCORBATE + / ImM PYRUVATE \or mM Mn*"^ â ^ or2mMOXALACETATE A \ \ + 5mM \ \~ ^*^ 1 1 V^
. Biological structure and function; proceedings. Biochemistry; Cytology. 58 F. EDMUND HUNTER, JR. just as they do other swelhng agents. Possibly they should be grouped with EDTA. As has been observed in other work Mn + + is considerably more effective than Mg + + in preserving mitochondrial structure. Because of 20 30 40 MINUTES Fig. 6. Inhibition of ascorbate-induced lysis of mitochondria by metal com- plexing agents. o CO ID < - - -â¢ââ¢â â¢â⢠« â¢v^ ASCORBATE + / ImM PYRUVATE \or mM Mn*"^ â ^ or2mMOXALACETATE A \ \ + 5mM \ \~ ^*^ 1 1 V^-r^^^^-r . 10 20 30 40 MINUTES 50 60 Fig. 7. Inhibition of ascorbate-induced lysis of mitochondria by Mg + +, Mn + +, pyruvate, and oxaloacetate. the inhibitory effect of 15 mM ascorbate, several possible reducing sub- stances were tested. Two mM nitrite has only a slight effect, but 20 mM frequently blocks completely for some time (Fig. 8). Hydroquinone and catechol completely prevent ascorbate-induced lysis in concentrations which have no effect or smaller effects on phosphate swelling. This may. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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