. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SIGNAL TRANSDUCTION IN CAP1TELH BY PKC 191 the PKC-like enzyme present in the Capitella lar\rae could he activated by JH-active compounds. Incubations were carried out using 100 ^g of the larval homogenates for IS inin at 30"C in the presence of either phosphatidylserine/ diolein (PS/DO). 10 ^M arachidonic acid (AA), 10 ^M JH III, 10 juA/ MF. or 10 MA/ elaidic acid (EA). The results of this experiment indicate that JH III, MF, and AA are able to activate CapiteUa PKC //; \-itn> (Fig. 5A). In comparison with activatio
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SIGNAL TRANSDUCTION IN CAP1TELH BY PKC 191 the PKC-like enzyme present in the Capitella lar\rae could he activated by JH-active compounds. Incubations were carried out using 100 ^g of the larval homogenates for IS inin at 30"C in the presence of either phosphatidylserine/ diolein (PS/DO). 10 ^M arachidonic acid (AA), 10 ^M JH III, 10 juA/ MF. or 10 MA/ elaidic acid (EA). The results of this experiment indicate that JH III, MF, and AA are able to activate CapiteUa PKC //; \-itn> (Fig. 5A). In comparison with activation by PS/DO, arachidonic acid was the stron- gest activator Wv RIM-1 To visualize the location of PKC in the CapiteUa larvae and juveniles and to identify possible chemosensory cells that would rapidly take up external chemicals or chemicals in the environment, larvae and juveniles were briefly (1 min) exposed to a fluorescently labeled protein kinase C inhibitor, rhodamine-conjugated bisindolylmaleimide (RIM-1), which has proven useful as a fluorescent probe for PKC (Chen and Poenie. 1993). After exposure to this in- hibitor, the larvae or juveniles were fixed, permeabilized. rinsed to remove excess unbound RIM-1. and viewed under a fluorescent microscope. In metatrochophore larvae, dis- tinct cells in the ciliary bands of the prototroch and telotroch 120 -f ° 100 - A i B. PS/DO Figure 5. Activation of an enzyme resembling protein kinase C (PKC) in CtipiteHa larval homogenates and purified rat brain PKC by JH-active chemicals. (A) Activation of the PKC-like enzyme present in larvae. PKC assays using Capitella larval homogenates were carried out in the presence of either phosphatidylserine/diolein (PS/DO). 10 \ arachidonic acid (AA). 10 /nA/ juvenile hormone (JH) III. 10 juiW trans, trans methyl farnesoate (MF). or 10 pM elaidic acid (EA). Incorporation of 32P into MBP4_I4 for AA. JH III. MF. and EA is shown expressed as the percentage incorporation relative to that found fo
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
