. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CYTOSKELETON IN A SYNCYTIAL SPONGE 249. Figure 6. Immunofluorescence of the microtubule cytoskeleton in adhered aggregates. (A) Six hours after plating, microtubule bundles al- ready stretch up to a centimeter across an entire coverslip. A-C, bar: 20 pm. (B) Microtubule bundles are rigidly straight (STR) in streams, while those leaving or converging on a stream (arrowheads) are often curved. (C) At the edges of preparations, bundles give way to a fine meshwork of microtubules that reach into lamellipodia (arrowheads). 7b). N
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CYTOSKELETON IN A SYNCYTIAL SPONGE 249. Figure 6. Immunofluorescence of the microtubule cytoskeleton in adhered aggregates. (A) Six hours after plating, microtubule bundles al- ready stretch up to a centimeter across an entire coverslip. A-C, bar: 20 pm. (B) Microtubule bundles are rigidly straight (STR) in streams, while those leaving or converging on a stream (arrowheads) are often curved. (C) At the edges of preparations, bundles give way to a fine meshwork of microtubules that reach into lamellipodia (arrowheads). 7b). Nuclei, mitochondria, coated vesicles, and tubulo- vesicular organelles were all found adjacent to microtubule bundles as well as lying free in the cytosol. Tracts of mi- crotubules ran beside, but not through, clusters of ar- chaeocytes and spherulous cells. In whole mounts of ag- gregates adhered to tissue extract on Formvar-coated gold EM grids, organelles of various sizes were also seen as- sociated with linear structures with diameters of approx- imately 22 nm, presumably microtubules (Fig. 7c). Inhibition experiments Organelle movement was reversibly inhibited by both nocodazole and colcemid but was unaffected by cyto- chalasin B (Table I), although the latter caused the tissue to detach from the substrate. Neither Taxol nor \Q nM EGTA had any effect on rate of organelle transport. Dye exchange Attempts to inject the fluorescent dyes carboxyfluores- cein or lucifer yellow through glass capillary microelec- trodes were not successful because of difficulty in obtaining stable penetrations. The surface membrane of adhered aggregates either blocked the electrode or did not reseal around the electrode after penetration. However, fluores- cent dyes coupled to acytoxymethyl esters such as Calcein AM (CAM) and Calcein blue AM (CBAM) could be readily loaded into dissociated tissue. Tissue loaded with CAM plated in the same culture dish with tissue loaded with CBAM produced fused ag
Size: 1008px × 2478px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
