Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . recentlyshown to be incorrect. A positive Nylander or Boettger test is probably due to thefollowing reactions: (a) Bi(OH)2N03 + KOH = Bi(OH)3 + KN03. (b) 2Bi(OH)3 — 30 = Bi2 + 3H20. 10. Fermentation Test. — Rubup in a mortar about 20 ofthe sugar solution with a smallpiece of compressed yeast. Transferthe mixture to a saccharometer(shown in Fig. 2) and stand it asidein a warm place for about twelvehours. If the sugar is fermentable,alcoholic fe


Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . recentlyshown to be incorrect. A positive Nylander or Boettger test is probably due to thefollowing reactions: (a) Bi(OH)2N03 + KOH = Bi(OH)3 + KN03. (b) 2Bi(OH)3 — 30 = Bi2 + 3H20. 10. Fermentation Test. — Rubup in a mortar about 20 ofthe sugar solution with a smallpiece of compressed yeast. Transferthe mixture to a saccharometer(shown in Fig. 2) and stand it asidein a warm place for about twelvehours. If the sugar is fermentable,alcoholic fermentation will occur andcarbon dioxide will collect as a gasin the upper portion of the the completion of fermentationintroduce a little potassium hydrox-ide solution into the graduated por-tion by means of a bent pipette,place the thumb tightly over theopening in the apparatus and invertthe saccharometer. Explain the re-sult. 1 Nylanders reagent is prepared by digesting 2 grams of bismuth snb-nitrate and 4 grams of Rochelle salt in 100 of a 10 per cent potas-sium hydroxide solution. The reagent is then cooled and Einiiorn Saccharometer. MONOSACCHARIDES. II ii. Barfoeds Test.—To -> 3 cc. of Barfoeds solution1 ina test-tube add a few drops of dextrose solution, and to stand a few moments and examine. Observe tbered precipitate. What is it? 12. Formation of Caramel.—Gently heat a small amountof pulverized dextrose in a test-tube. After the sugar hasmelted and turned brown, allow the tube to cool, add waterand warm. The coloring matter produced is known ascaramel. 13. Demonstration of Optical Activity.—A demonstra-tion of the use of the polariscope, by the instructor, each stu-dent being required to take readings and compute the spe-cific rotation. Use of the Polariscope. For a detailed description of the different forms of polari-scopes, the method of manipulation and the principles in-volved the student is referred to any standard


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