. Animal micrology; practical exercises in microscopical methods. Microscopy -- Technique; Microscopes -- Technique. Chapter IV: Simple Section Methods 35 of paraffin. As the paraffin melts transfer drops of the edge of the tissue, which has been previously placed on the cork. The paraffin cools and holds the tissue fast. Another and better method of handling a small object is to imbed it in a piece of hardened liver. In sectioning, the liver as well as the object is sliced, but they readily separate when placed in alcohol. Beef liver or dog liver is prepared for such purposes by hardeni
. Animal micrology; practical exercises in microscopical methods. Microscopy -- Technique; Microscopes -- Technique. Chapter IV: Simple Section Methods 35 of paraffin. As the paraffin melts transfer drops of the edge of the tissue, which has been previously placed on the cork. The paraffin cools and holds the tissue fast. Another and better method of handling a small object is to imbed it in a piece of hardened liver. In sectioning, the liver as well as the object is sliced, but they readily separate when placed in alcohol. Beef liver or dog liver is prepared for such purposes by hardening pieces about 5x2x2 cm. in size in 95 per cent, alcohol for 24 hours, and then trans- ferring to fresh 95 per cent, alcohol until needed. When much hand sectioning is to be done, a supply of hardened liver should be kept on hand. Many small objects may be held between pieces of pith, and successfully sectioned. 3. Well Microtomes (Fig. 27) are inexpensive instruments which are used for simple sectioning. Such a microtome consists of a tube in which the object is placed, and at one end of which is a plate to guide the razor. The other end is provided with a screw, which, when turned, pushes the contents of the tube above the plate, thus making it possible to cut sections of a uni- form thickness. The object to be cut must be firmly fixed in the well. Such tissues as kidney, liver, spleen, hard tumors, cartilage, etc., may be held sufficiently rigid by wedging small slabs of carrot, turnip, pith, or hardened liver in about them. These supporting substances must, of course, rest squarely against the bottom of the well. Soft tissues, such as soft tumors or brain, must be imbedded. Three parts of paraffin and one part of vaselin melted together and thoroughly mixed makes a very good imbedding-mass for a well microtome. To imbed, warm the microtome slightly and fill the well with the imbedding mix- ture. Remove all liquid from the surface of the tissue, and pass it below the surfac
Size: 1067px × 2341px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookpublisherchicagotheuniversityofchicagopress, bookyear1906
