. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . i8 LABORATORY GUIDE IN BACTERIOLOGY filtered rapidly by the use of suitable apparatus, as illus-trated in Fig. lo. At the connection with the vacuumpump a valve should be inserted—or a flask with a rub-. FlG. lo Filtering Media by Means of Vacuum Pumpa. Liquid medium c. Reiiex flaslc 6. Absorbent cotton /. Rubber stopper with two holes c. Rubber stopper g. Connection with aspirator d. Filtered medium ber cork with two holes may take its place—to preventthe water from entering the flask. Whichever metho
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . i8 LABORATORY GUIDE IN BACTERIOLOGY filtered rapidly by the use of suitable apparatus, as illus-trated in Fig. lo. At the connection with the vacuumpump a valve should be inserted—or a flask with a rub-. FlG. lo Filtering Media by Means of Vacuum Pumpa. Liquid medium c. Reiiex flaslc 6. Absorbent cotton /. Rubber stopper with two holes c. Rubber stopper g. Connection with aspirator d. Filtered medium ber cork with two holes may take its place—to preventthe water from entering the flask. Whichever method is employed, the filter must alwaysfirst be soaked in hot water, or, better, kept in steamuntil used. PREPARATION OF CULTURE MEDIA 19 The filtered liquid is collected in a smaller funnel(Fig- S> 0- A wire basket should be supported so as tobe inclined to an angle of about 45°, so as to allow theproper arrangement of the tubes. Remove the plug of a cuhure-tube and quickly passthe latter up the full length of the pipette (Fig. 5, /), whichshould be no less than 3 inches long. Open the pinch-cock, fill the tube one-third full, and then quickly with-draw the tube so as toleave no trace of the me-dium on the upper part ofthe tube, and replace thecotton stopper. If thisprecaution is ne
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