. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 6 Hours Fig. 4. Changes in P/ml, i?/ml, RNA/ml, and protein/ml in suspensions of lysogenic megatheyiuin Sgqa in 5% peptone kept in log growth by repeated dilution. Cells from 18 h 5% peptone slant washed in 5% peptone, diluted to I • lo^B/ml. Stand 25°, 18 h and then shake at 34*^. Suspension diluted 1/5 with fresh 5% peptone as soon as B/ml = 500-10*.. Fig. 5. Changes in free phage and Bjml in suspensions of megathermin sensitive + phage T. Cell
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 6 Hours Fig. 4. Changes in P/ml, i?/ml, RNA/ml, and protein/ml in suspensions of lysogenic megatheyiuin Sgqa in 5% peptone kept in log growth by repeated dilution. Cells from 18 h 5% peptone slant washed in 5% peptone, diluted to I • lo^B/ml. Stand 25°, 18 h and then shake at 34*^. Suspension diluted 1/5 with fresh 5% peptone as soon as B/ml = 500-10*.. Fig. 5. Changes in free phage and Bjml in suspensions of megathermin sensitive + phage T. Cells from 18 h 5% peptone agar slant washed off in 5% peptone and dilu- ted to 4-10' cells/ml. 80 "T" phage particles/ml added and then tubes shaken at 34°. Suspension diluted 1/5 when S/ml = 2-10*. all the successive growth steps. The RNA and protein growth rates are also equal to the cell and phage growth rates. In other words, the composition of the cells remained exactly the same with respect to phage, protein and RNA throughout the experiment and would continue to do so indefinitely until some change in the media occurred, or a new type of cell appeared. The fact that such a constant ratio of P/B would result in a lysogenic culture was pointed out by Delbruck". Rate o\ phage production and cell groiuth with sensitive cells The results of an experiment in which T phage was added to a culture oimegatheriiim sensitive, growing in 5% peptone, are shown in I'ig. 5. In tins case, the phage growth rate remains at its original higli value throughout, and as a result the ratio of PjB continues to increase very rapidly. When this ratio reaches about 50/1, lysis occurs. Lysis caused by such high PjB ratios is not accompanied by an increase in the number of phage particles (Northrop and Krueger^), (Northrop'^), (Delbruck*'). This explains the fact that there is no change in slope of the P curve when lysis starts. References p. Please note that these images are ext
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