. Biological transport. Biological transport; Biological Transport. BIOLOGICAL TRANSPORT be relieved by free histidine, Stein (1958) argued that the binding site could not be a sulfhydryl group, as had been suggested. Instead he has reported in a preliminary paper (Stein, 1958) the detection of an N-terminal histidine whose reaction with phenylisothiocyanate can be specifically blocked by the presence of 1,3-propanediol. If this approach proves to have broad applicability, it should help very much in the identification of transport sites and the discovery of their function. A series of fixed s
. Biological transport. Biological transport; Biological Transport. BIOLOGICAL TRANSPORT be relieved by free histidine, Stein (1958) argued that the binding site could not be a sulfhydryl group, as had been suggested. Instead he has reported in a preliminary paper (Stein, 1958) the detection of an N-terminal histidine whose reaction with phenylisothiocyanate can be specifically blocked by the presence of 1,3-propanediol. If this approach proves to have broad applicability, it should help very much in the identification of transport sites and the discovery of their function. A series of fixed sites leading through the membrane and re- ceiving the solute in turn, one from the other, with complete re- versibility should yield the same behavior as a single fixed site. Fig- ure 8 shows Danielli's visualization of a polar pore in a lipid barrier through which a solute could migrate, by hydrogen bonding, across the interval between pairs or sets of polar groups. Danielli (see the quotation, page 28) also considered the possibility that oscillations Protein molecule Lipoid molecule. Polar pore Figure 8 Diagram of a polar pore in a lipid membrane, as visualized by Danielli. The pore consists of a succession of polar sites on polypep- tide chains extending through the bimolecular layer of lipid. \Fro?n Danielli, J. F. (1954), Proc. Symposium Colston Research Soc, 7, 1; with permission.'] 20. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Christensen, Halvor N. New York, W. A. Benjamin
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