. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ASCID1AN HEMOCYTE AGGREGATION 251. Untreated pi. Acid-treated pi. Alkali-treated pi. Heat-treated pi. Dialyzed pi. Intact pi. Dialyzed pi. Intact pi. + trypsin Dialyzed pi. + trypsin Intact untreated pi. Reacted pi. 50 100 150 200 AGGREGATION (%) Figure 5. Effects of various preparations of plasma on the aggregation of hemocytes of Halocynthia roretzi. (a) Intact plasma was previously treated with acid, alkali, or heat (90°C), or was dialyzed, and effects of these preparations (10%) on the aggregation were measured by method
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ASCID1AN HEMOCYTE AGGREGATION 251. Untreated pi. Acid-treated pi. Alkali-treated pi. Heat-treated pi. Dialyzed pi. Intact pi. Dialyzed pi. Intact pi. + trypsin Dialyzed pi. + trypsin Intact untreated pi. Reacted pi. 50 100 150 200 AGGREGATION (%) Figure 5. Effects of various preparations of plasma on the aggregation of hemocytes of Halocynthia roretzi. (a) Intact plasma was previously treated with acid, alkali, or heat (90°C), or was dialyzed, and effects of these preparations (10%) on the aggregation were measured by method B. (b) Intact plasma or dialyzed plasma was treated with or without trypsin ( mg/ml) at 20°C for 4 h and filtered through a Centncut membrane (10,000 molecular weight cut-off). The effects of the resulting nitrates on the aggregation were measured by method B. (c) The effect of reacted plasma on the aggregation was measured by method B. Reacted plasma was collected from hemolymph in which the aggregation of he- mocytes had been completed. The results shown are means of relative values of triplicate tests (±SD); the aggregation induced by 2 mAf MgCK was defined as 100%. Note that an approximately linear relationship exists between the extent of aggregation and the concentration of plasma factor tested in this experiment. plasma (Fig. 5c), which suggests that one or more inducing substances are produced when the aggregation of hemo- cytes takes place in hemolymph. Inducing effects of Met-Lys-bradykinin on hemocyte aggregation Stefano el al. (1989a, b) have reported that Met-en- kephalin triggers inflammatory responses from the hemo- cytes of the mollusk Mytilus edulis by inducing their morphological change, migration, and aggregation. To investigate whether neuropeptides, such as Met-enkeph- alin, would induce ascidian hemocytes to aggregate, we used method B to examine the effects of 26 biologically active peptides on the aggregation of//, rorelzi hemocytes. Typical resul
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