. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Injection Altered Patterns Figure 1. Experimental alteration of cell lineages in ilie nemertean Cerebratulus lacteus. (a) Procedure employed to shift the plane of the first cleavage division. Fertilized eggs were compressed to orient the spindle parallel to the plane of compression. After first cleavage, the coverslip was removed and one blastomere was injected with a fluorescent lineage tracer at the two-cell stage, (b) Diagrams showing left lateral and apical views of the pilidium larva. The dorsal side is to the right, an
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Injection Altered Patterns Figure 1. Experimental alteration of cell lineages in ilie nemertean Cerebratulus lacteus. (a) Procedure employed to shift the plane of the first cleavage division. Fertilized eggs were compressed to orient the spindle parallel to the plane of compression. After first cleavage, the coverslip was removed and one blastomere was injected with a fluorescent lineage tracer at the two-cell stage, (b) Diagrams showing left lateral and apical views of the pilidium larva. The dorsal side is to the right, and the ventral side to the left, (c) Normal and altered labeling patterns observed in this study (apical views shown). The line dividing the larvae in half corresponds to the boundary between labeled and unlabeled eclodermal domains, and thus to the hr\i cleavage plane, \umbers indicate the number of cases displaying each of the indicated labeling patients. L. left: R. right: D. dorsal: I', ventral: L \ 'O, left ventral oblique: RDO. right dorsal oblique: RI 'O. right ventral oblique: LDO, left dorsal oblique, labeling patterns. A wide range of oblique labeling patterns was actually observed: but they are lumped into four general categories here for the sake of simplicity, (d) Combined DIC and fluorescence micrographs showing one ol the four normal labeling patterns (R. right), in which the first cleavage plane corresponds to the plane of bilateral symmetry. I 'iew is from the oral pole, opposite the apical organ. Line demarcates the labeled and unlabeled eclodermal domains. Arrows mark the plane of bilateral symmetry. Dorsal side is to the right, gt. gut. le) A n altered labeling pattern IRI'O. right ventral oblique), in which l/ic tir\t cleavage plane passed obliquely relative to the plane of bilateral symmetry mid the frontal plane. Scale bar equals 50 nm. and subsequent development to the pilidium larva (Fig. Ib) were normal. Forty-one of these cases displayed one of the fou
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
