. A laboratory guide in elementary bacteriology. Bacteriology. 20 General Bacteriology. 1 gm. 2 gm. 300 cc. 2 gms. 25 cc. 75 This stain should stand 24 hours and then be filtered. It does not keep well and must not be used when more than 14 days old. 6. Gram's Iodine solution. Iodine, - . - - - Potassium iodide, . - - Distilled water, . - - - - 7. Gabbett's Methylen blue solution. Methylen blue (dry), - - - - Sulphuric acid, - . - - Distilled water, . . 8. Alcohol, 96%. References. A. 156; H. 75; M. & W. 245; M. & R. 103; McF. 90; P. 200. Special Directions. Prepare the solutions
. A laboratory guide in elementary bacteriology. Bacteriology. 20 General Bacteriology. 1 gm. 2 gm. 300 cc. 2 gms. 25 cc. 75 This stain should stand 24 hours and then be filtered. It does not keep well and must not be used when more than 14 days old. 6. Gram's Iodine solution. Iodine, - . - - - Potassium iodide, . - - Distilled water, . - - - - 7. Gabbett's Methylen blue solution. Methylen blue (dry), - - - - Sulphuric acid, - . - - Distilled water, . . 8. Alcohol, 96%. References. A. 156; H. 75; M. & W. 245; M. & R. 103; McF. 90; P. 200. Special Directions. Prepare the solutions of dyes from the saturated alcoholic solutions (furnished) and place them in 2 oz. bottles arranged with pipettes and neatly labeled. The bottles are conveniently kept in a block. Fig. 8. EXERCISE XVII. SIMPLE COVER-QLASS PREPARATION. p,^ g Block for stain bottles. General Directions. Bacteria may be studied under the microscope in a living condition in a hanging drop preparation (XIX); but on account of their hyaline charac- ter, which makes the examination difficult, the student should first learn to stain them and later make the hanging drop preparation. With a few exceptions all bacteria can be stained by the following process: A small drop of distilled water is placed on a clean cover-glass by means of the platinum loop. With a sterile needle a portion of the material to be examined is secured and while the cover-glass is held in the fingers of the left hand the bacteria on the needle are introduced into the water, thoroughly mixed and then spread in a thin film over as much of the surface of the cover-glass as possible. When the bacteria are taken from fluid media a drop of water will not be necessary. In this case use a loop. The film is now allowed to dry. If the drop is sufficiently small this will be a short process. It may be hastened by holding the cover-glass high over the flame, but it should always be held in the hand to prevent over-heating, which spoils the pre
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