. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1234 —- - 5 6. 45- 29- Figure 2. Immunologic demonstration ofurmacroglobulin in Limw/tis blood cells and in the materials released dunng degranulation of the cells. Alpha:-macroglobulin is evident in the plasma (lanes and ) and whole, undegranulated cells (lanes , and ) hut is not demonstrable in the penultimate wash of the cells (lanes and ). Alpha;-macroglobulin is also demonstrable in the materials released from the cells during degranulation (lanes and ) but not in the degranulated cells (l
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1234 —- - 5 6. 45- 29- Figure 2. Immunologic demonstration ofurmacroglobulin in Limw/tis blood cells and in the materials released dunng degranulation of the cells. Alpha:-macroglobulin is evident in the plasma (lanes and ) and whole, undegranulated cells (lanes , and ) hut is not demonstrable in the penultimate wash of the cells (lanes and ). Alpha;-macroglobulin is also demonstrable in the materials released from the cells during degranulation (lanes and ) but not in the degranulated cells (lanes and ). Lane B. 1 was loaded with jig of purified «i-macroglobulin. Lanes and contained M! of plasma; lane contained 2 p\ of pelleted live, whole blood cells; lane contained 1 ^1 of pelleted whole, live blood cells; lanes and contained 10 M' of buffer from the penultimate cell wash; and lanes and contained 10/il of releasate. ). indicating that most or all of the cell-associated «:- macroglobulin had been released during exocytosis. The apparent molecular weight of the subunit of cell-associ- ated rt:-macroglobulin was identical to that of the plasma form of «:-macroglobulin. The relative amounts of pro- tein in different bands of Coomassie blue-stained gels were estimated spectrophotometrically with the curve in- tegration function of a Bio Rad Model 620 scanning den- sitometer. Estimations of the relative amounts of «2- macroglobulin by the optical density of the 185 kDa band on Coomassie blue-stained gels of whole cells and plasma indicate that the cells contained in a given vol- ume of whole blood contain approximately of the a:-macroglobulin contained in the same volume of plasma. Measurement activity The ability to protect trypsin from inactivation by soy- bean trypsin inhibitor was used to estimate the amounts of active a2-macroglobulin in samples of plasma and the materia
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
