American journal of physiology . thereagents used such was found to be the case. Paramoecium aurelium.—The structural changes caused by lack ofoxygen have been described by Loeb and Hardesty; ^ but their ex-periments have been repeated in the present series for the sake ofcomparison. A few drops of Paramoecium culture were enclosed inan Engelmann gas chamber, through which was passed hydrogenthat had been washed in potassium hydrate solution, potassium per-manganate solution, and water. After the stream of hydrogenhas been passing for several hours,the time varying with the temp-erature of the
American journal of physiology . thereagents used such was found to be the case. Paramoecium aurelium.—The structural changes caused by lack ofoxygen have been described by Loeb and Hardesty; ^ but their ex-periments have been repeated in the present series for the sake ofcomparison. A few drops of Paramoecium culture were enclosed inan Engelmann gas chamber, through which was passed hydrogenthat had been washed in potassium hydrate solution, potassium per-manganate solution, and water. After the stream of hydrogenhas been passing for several hours,the time varying with the temp-erature of the room, the animalsbegin to swim more slowly, theyabsorb water, the contractile vacu-oles increase in size, or severaladditional vacuoles may appear, and they sink to the bottom. Oneor more clear vesicles now protrude from the surface of the animal, ^ Araki : Zeitschr. f. physiol. Chemie, 1891, xv, p. 325. ^ RiCHET: Archives de physiologic, 1876, viii, p. 829. 3 Loeb and Hardesty: Archiv f. d. ges. Physiol., 1895, ixi, p. Figure i. 212 Sidney P. Bndgett. and into these some of the vacuoles usually escape ; the vesicles finallybiust, and the cell contents are extruded (Fig. i). If a drop of a per cent solution of potassium cyanide be addedto several drops of Paramoecium culture, there result structural changes which are apparentlyiM\. exactly similar to those de-rT scribed above, but they occurimmediately (Fig. 2). If thesolution be more concentrated,for instance per cent, thebursting often occurs at once, without a preceding marked change ofform. Amoeba. — When deprived of oxygen. Amoeba becomes vacuolatedand tends to assume a spherical form (Fig. 3). A dilute solution ofantipyrine produces the same effects (Fig. 4). The same is true ofdigitaline and potassium cyanide solutions.
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