. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 8 10 12 PH Fig. 3. Effect of pH on the pre- cipitation of the 3 :4-5 :6-dibenz- carbazole-caffeine cone, of dibenzcarbazole in M caffeine before precipitation of the complex; F^ cone, of dibenz- carbazole in M caffeine after precipitation of the complex; F^- F^, dibenzcarbazole precipitated as complex. 300- f â Q C>l00i. -â -o- 3 Time (hours) Fig. 4. Effect of purine cone, on precipitation of 3
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 8 10 12 PH Fig. 3. Effect of pH on the pre- cipitation of the 3 :4-5 :6-dibenz- carbazole-caffeine cone, of dibenzcarbazole in M caffeine before precipitation of the complex; F^ cone, of dibenz- carbazole in M caffeine after precipitation of the complex; F^- F^, dibenzcarbazole precipitated as complex. 300- f â Q C>l00i. -â -o- 3 Time (hours) Fig. 4. Effect of purine cone, on precipitation of 3:4-5:6- dibenzocarbazole-caffeine complex. ® (S), cone, of di- benzcarbazole in M caffeine; x X, in M caffeine; O -O, in ^^ caffeine. Effect of purine concentration on the precipitation of the 3:4-5:6-dibenzcarbazole- caffeine complex. The concentration of dibenzcarbazole at various intervals of time after mixing was estimated, using , and ^^ solutions of caffeine. The results showed that the maximum concentration of dibenzcarbazole, the amount of complex precipitated and the dibenzcarbazole remaining in solution when the equilibrium had been reached increased with increase of caffeine concentration. The time required to reach the maximum concentration of dibenzcarbazole and to reach equilibrium however was not affected (Fig. 4). Effect of amount of dibenzcarbazole added on the precipitation of the complex. In these experiments suspensions of 20, 50, 100 and 200 mg/1 of 3:4-5:6-dibenzcarbazole in M caffeine solution were examined. The more concentrated suspensions produced a greater maximum concentration of dibenzcarbazole in solution before precipitation of the complex and a shorter time was required for the equilibrium to be reached. Similarly, by reducing the amount of dibenzcarbazole added to a M solution of tetramethyluric acid from 200 mg/1, when the maximum dibenzcarbazole concen- tration was reached too quickly for detecti
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