. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LEUCOPHORE-ACTIVATING SUBSTANCES 207 volume, but the activity appeared later and peaked in fraction 12. Thus, by treat- ment with chloroform or acetone it was possible to remove the white pigment- dispersing hormone from the heavy component in the ethanol-soluble material. The aim of the next experiment was to determine the effect of boiling on the white pigment-dispersing substances in an extract prepared directly in water and in the ethanol-soluble fraction of the eyestalks of Uca and of Palaemonetes as measured by changes
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LEUCOPHORE-ACTIVATING SUBSTANCES 207 volume, but the activity appeared later and peaked in fraction 12. Thus, by treat- ment with chloroform or acetone it was possible to remove the white pigment- dispersing hormone from the heavy component in the ethanol-soluble material. The aim of the next experiment was to determine the effect of boiling on the white pigment-dispersing substances in an extract prepared directly in water and in the ethanol-soluble fraction of the eyestalks of Uca and of Palaemonetes as measured by changes in the responses to these substances. Distilled water ex- 25 20 15 10 0. 7 8 9 10 FRACTION I 12 13 14 NUMBER 6 FIGURE 5. The white pigment-dispersing Standard Integrated Responses (SIR) evoked by the various fractions of the distilled water eluates of the ethanol-soluble fraction prepared from fresh eyestalks of Uca (dots) and of the distilled water eluates of the ethanol-soluble fraction prepared from the eyestalks of Uca that were pre-extracted with acetone (circles) or chloroform (half-filled circles) after filtration through a column of Bio-Gel P-6. Other details same as Figure 3. tracts were made from 50 eyestalks of Palaemonetes and 50 eyestalks of Uca. The extracts were filtered individually through the column of Bio-Gel P-6 and frac- tion 12, which produced the most white pigment-dispersing activity of the material retarded by the gel (Figs. 3, 4, 5), was divided into two equal portions. One portion of the extract was placed in a boiling water bath for five minutes while the other portion was left at room temperature. The boiled extract was cooled, made up to the original volume, and then the boiled and unboiled extracts were each assayed for white pigment-dispersing activity on 10 intact crabs adapted to. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illus
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
