The Biochemical journal, 1907 . variably causesleakage. The osmometer is screwed together by means of a male screwon the lower casing and a female screw on a circular joining piece ofthick metal possessing a flange, which fits against a flange on the uppercase as shown in the drawing. The thumb hold on the lower caseenables the whole to be tightly screwed together. The capacity of each chamber was about 20 , the diameter5 cm., and the depth i cm. ; so that a large surface for diffusionand rapid equalization of crystalloids was offered, relatively, to thevolumes of the chambers. In fitting u
The Biochemical journal, 1907 . variably causesleakage. The osmometer is screwed together by means of a male screwon the lower casing and a female screw on a circular joining piece ofthick metal possessing a flange, which fits against a flange on the uppercase as shown in the drawing. The thumb hold on the lower caseenables the whole to be tightly screwed together. The capacity of each chamber was about 20 , the diameter5 cm., and the depth i cm. ; so that a large surface for diffusionand rapid equalization of crystalloids was offered, relatively, to thevolumes of the chambers. In fitting up the osmometer a circle of parchment paper, the OSMOTIC PRESSIRK OF COi,I,()II)\L SOLUTIONS 49 exact size of the platinum grid, is cut out from a sheet, thoroughlywetted, and applied to the grid, which is then placed in position andthe apparatus screwed tight. Any leakage is then tested for by puttingthe osmometer in a vessel of water, blowing air in, and observingwhether any air bubbles appear. In order to prevent blowing the. Fig. 2. Photograph of osmometer fitted up and joined to manometer. membrane away from the platinum grid, the two outlet tubes ofplatinum may be joined, by rubber tubing, to a glass T piece, andblown up together, as any leakage is usually to the outside andnot from one chamber to the other, good parchment paper in sheetbeing remarkably free from imperfections. 50 BIO-CHEMICAL JOURNAL The next step is the filling of the osmometer. The chamberon the upper, or colloid, side is first filled with the solution, in whichthe pressure of colloid is to be measured, by means of a narrow tubedpipette, and the proximal limb of the mercurial manometer, whichis of narrow^ bore, as also the connections and funnel on the manometerside (see Fig. 2) having been previously filled with the solution, orvvith oil, and clipped oft at the end to be connected to the osmometer,the osmometer and tubing are joined. Care should be taken to avoidinclusion of air in making the connecti
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