. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MOTILITY AND AGING OF ARBACIA SPERM x JOSEPH M. BRANHAM 2 Marine Biological Laboratory, Woods Hole, Massachusetts 02543 It is well known that diluted sea urchin sperm have a relatively short effective life span. In a few hours or days they lose the ability to activate eggs, become immotile and their respiration ceases (Gemmill, 1900; Cohn, 1918; Gray, 1928, 1931; Rothschild, 1951; Tyler, 1953; Rothschild and Tyler, 1954; Bishop, 1962; Mann, 1964). The loss of vitality of sperm is reported to be associated with the exhaustion
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MOTILITY AND AGING OF ARBACIA SPERM x JOSEPH M. BRANHAM 2 Marine Biological Laboratory, Woods Hole, Massachusetts 02543 It is well known that diluted sea urchin sperm have a relatively short effective life span. In a few hours or days they lose the ability to activate eggs, become immotile and their respiration ceases (Gemmill, 1900; Cohn, 1918; Gray, 1928, 1931; Rothschild, 1951; Tyler, 1953; Rothschild and Tyler, 1954; Bishop, 1962; Mann, 1964). The loss of vitality of sperm is reported to be associated with the exhaustion of energy reserves (Gemmill, 1900; Cohn, 1918; Tyler, 1953). Gemmill recog- nized that sperm were more active, and also pointed out (p. 171) that "on com- paring the movement of spermatozoa in different mixtures [dilutions], one finds that the difference in activity is not sufficiently marked to account for the very early loss of vitality of spermatozoa in the weaker mixtures simply in terms of exhaustion of ; The relationship between the motility of sea urchin sperm and the rate at which they lose the ability to activate eggs remains somewhat un- certain, primarily because it is difficult to evaluate quantitatively the motility of spermatozoa. The problems of finding the motility status of semen samples are manifold. Microscopic examination to determine sperm activity involves many variables that are difficult to control (Bishop, 1962; Rikmenspoel, 1962; Rothschild, 1953; van Duijn, 1963, 1964). The impedance change frequency method for rating motility (Rothschild 1948a) allows better control of these variables but is unsuitable with diluted semen. A simple method of rating sperm motility which avoids some of these problems was devised for this study. The first part of this report deals with an analysis of the method for rating motility. The second part is an investigation of senescence of sperm, utilizing the method for rating motility. The following results
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
