. Pathological technique; a practical manual for workers in pathological histology and bacteriology. -agar. 1 There is no trustworthy evidence that any other Gram-decolorizing micro-coccus than the gonococcus ever occurs in gonorrheal pus. F. T. Lord, work-ing in the laboratory of the Massachusetts General Hospital, examined by cul-tures the pus from 22 cases of gonorrhea, and in none could he find anyGram-decolorizing micrococcus other than the gonococcus. PATHOGENIC BACTERIA AND FUNGI. 275 In making transplants to plain agar avoid carrying overany of the albuminous material of the special cu
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. -agar. 1 There is no trustworthy evidence that any other Gram-decolorizing micro-coccus than the gonococcus ever occurs in gonorrheal pus. F. T. Lord, work-ing in the laboratory of the Massachusetts General Hospital, examined by cul-tures the pus from 22 cases of gonorrhea, and in none could he find anyGram-decolorizing micrococcus other than the gonococcus. PATHOGENIC BACTERIA AND FUNGI. 275 In making transplants to plain agar avoid carrying overany of the albuminous material of the special culture-medium, for this material may permit some growth of thegonococcus on the plain agar. In applying the test of decolorization by Grams method,colonies not more than forty-eight hours old should be used,because Gram-staining cocci in older cultures may be moreor less decolorized by this method. In proof of the neces-sity of cultures for confirming the identity of the gonococcusin certain instances we may state that we have met with aGram-decolorizing coccus in an arthritis of the knee, clini-. FiG. 49.—Gonococci inside a leucocyte. Cover-glass preparation from gonor-rheal pus ; X 2000 (Wright and Brown). cally of gonorrheal origin, which, in cover-glass preparationsfrom the exudate, was regarded as the gonococcus, but whichwas found not to be that organism by the study of it incultures. If it is desired to obtain cultures of the gonococcus fromthe pus of gonorrheal urethritis, the case should not be morethan of a few days duration, because cases of longer dura-tion will usually show the presence of other bacteria whosecolonies overgrow the feebly growing colonies of the gono-coccus. An organism which may be mistaken for the gono-coccus is a coccus growing in large milk-white colonies onall media, but staining by Grams method. This coccus is 276 PA THOL O GICA L TE CHNIQ UE. frequently found in gonorrheal pus after the discharge haslasted several days. Other cocci also occu
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