. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIPOPROTEIN IN DEVELOPING BLUE CRAB. Figure 2. [mmunohistochemical study using anti-LpI Hematoxylin counterstain; original magnifications X1000; bar = 20 /urn), (a) Stage 4 embryo: Neither the embryonic cell mass nor the adjacent yolk (y) demonstrates immunoreactivity for Lpl. (b) Stage 7 embryo: Focal immunoprecipitates decorate the yolk (y) and are also localized at the interface of the lateral cuboidal cells (cb) with the yolk (arrows), (c) Stage 10 embryo (protozoea): The yolk (y) has been reduced to small bilateral mass
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIPOPROTEIN IN DEVELOPING BLUE CRAB. Figure 2. [mmunohistochemical study using anti-LpI Hematoxylin counterstain; original magnifications X1000; bar = 20 /urn), (a) Stage 4 embryo: Neither the embryonic cell mass nor the adjacent yolk (y) demonstrates immunoreactivity for Lpl. (b) Stage 7 embryo: Focal immunoprecipitates decorate the yolk (y) and are also localized at the interface of the lateral cuboidal cells (cb) with the yolk (arrows), (c) Stage 10 embryo (protozoea): The yolk (y) has been reduced to small bilateral masses surrounded by cuboidal cells. One such mass is delineated in this image. There are immunoprecipitates within the yolk and within the cytoplasm of the cuboidal cells, (d) Hepatopancreas of megalope: The hepatopancreas is composed of tubules of cuboidal and low columnar cells. Immunoprecipitates are concentrated at the apices, (e) Gill of megalope: The hemolymph within the vascular spaces is strongly immunoreactive for Lpl, while the background tissue elements are nonreactive. (f) Adult hepatopancreas: Immunoreactivity for Lpl is concentrated in the cytoplasm of the vacuolated (R and B) cells. In this preparation, the cytoplasm of the F cells is obscured by the more intense cytoplasmic reactivity of the R and B cells, but their nuclei (F) can still be seen. assumed to be the anlagen of the hepatopancreas. Anti-LpI immunoreactivity was present within the yolk residue and could also be seen in the cytoplasm of the cuboidal cells (Fig. 2c). Lan'ae. The hepatopancreas of the megalopae consisted of a collection of tubular glands, less complex than the adult hepatopancreas. The glands were lined by a single layer of epithelium surrounded by a basement membrane. The lining cells were cuboidal to low columnar in shape and had basophilic cytoplasm. They resembled the anti-LpI-reactive cuboidal cells observed in late embryo stages. The nuclei were centrally located in some cells and bas
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology