. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REGENERATION INHIBITORS IN TUBULARIA 85 to the slightest extent during plugging was not used for experimental purposes. The stems were placed in V-shaped grooves cut in a tray of 2% agar gel made with filtered pasteurized sea water containing chloromycetin (100 ). They were oriented so that any charged material in the plugs would be drawn into the animal when a current was applied (Fig. 2). (Pilot experiments had revealed that the plugged stems had to be placed in an electric current for the primordial extract gel to i
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REGENERATION INHIBITORS IN TUBULARIA 85 to the slightest extent during plugging was not used for experimental purposes. The stems were placed in V-shaped grooves cut in a tray of 2% agar gel made with filtered pasteurized sea water containing chloromycetin (100 ). They were oriented so that any charged material in the plugs would be drawn into the animal when a current was applied (Fig. 2). (Pilot experiments had revealed that the plugged stems had to be placed in an electric current for the primordial extract gel to inhibit the rate of regeneration.) A series of experiments was conducted to determine the current to which Tubularia could be subjected for 8 hours and still undergo typical regeneration. A current of 1 caused slowing of the rate of regeneration and some reversible damage to the stems. A current of 1 slightly retarded the rate of reconstitution. The current used in these experiments (1 ). T T, e FIGURE 3. Tubularia in agar tray exposed to an electric field (6 volts; 10 milliamperes) for 8 hours, e, platinum electrode; , filtered pasteurized sea water containing chloromy- cetin; /./>., filter paper wicks; T, Tubularia stems in grooves cut in tray of agar; , siphon tube. did not visibly damage the stems and allowed typical regeneration. There was no evidence of the reversal of polarity which has been reported when higher currents were applied over longer periods (Barth, 1934; Levin, 1961). A plastic tray (173 X 85 X 8 mm.) containing plugged Tubularia and a group of unplugged stems was placed in an electric current of 10 milliamperes and a voltage of 6 for eight hours (Fig. 3). The temperature was maintained at 15-16° C. during the entire eight hours. The stems were under a constant flow (100 hr.) of filtered pasteurized sea water containing chloromycetin (100 ) This. Please note that these images are extract
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
