. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 50 P. L. DEFUR ET AL 60 I 40 20 (torr). PH "so o o Figure 1. Oxygen binding by stripped He of normoxic (O), 7 days hypoxic (•). and 25 day hypoxic (A) blue crabs. 25°C, M Tns maleate buffered saline containing 494 mM NaCl, 16 mM KC1. 23 mA/ CaCI:. 23 mA/ MgCl, 25 mA/(Na):SO4 and 2 mM NaHCO,. Upper panel shows oxygen affinity (PsuK and lower panel shows the coopera- tivityat PCK = Pw (n,,,). live procedure for data analysis is available. In the lower panel of F
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 50 P. L. DEFUR ET AL 60 I 40 20 (torr). PH "so o o Figure 1. Oxygen binding by stripped He of normoxic (O), 7 days hypoxic (•). and 25 day hypoxic (A) blue crabs. 25°C, M Tns maleate buffered saline containing 494 mM NaCl, 16 mM KC1. 23 mA/ CaCI:. 23 mA/ MgCl, 25 mA/(Na):SO4 and 2 mM NaHCO,. Upper panel shows oxygen affinity (PsuK and lower panel shows the coopera- tivityat PCK = Pw (n,,,). live procedure for data analysis is available. In the lower panel of Figure 1, cooperativity seems to decrease at 7 days, but the mean values are not significantly different (P = .10). A Mann-Whitney U test also did not distin- guish a significant change. There was a clear change in the subunit composition of the Hcs (Fig. 2). Specifically, subunits 3, 5, and 6 de- creased in concentration relative to subunit 4, which has remained invariant in samples taken, by now, from more than 500 individuals (Mangum, unpubl. obs.; Rainer, 1988). After 25 days of hypoxia, HcO: affinity increased fur- ther (Fig. 1). The 95% confidence interval around a re- gression line describing O: affinity does not overlap those for control or 7-day hypoxic animals in any part of the pH range (Table II). The slope of the regression line de- scribing the 25 day data ( ± 95% ) does not differ from the other two. In this case, the mean value for cooperativity ( ± ) of the He from 25 day hypoxic animals (Fig. 1) differs significantly (P= .02) from that for control and 7 day hypoxic animals ( ±). All three variable subunits decreased further in con- centration relative to subunit 4 (Fig. 2). Indeed the pres- ence of subunit 3, which is sometimes completely absent (Mason et ai. 1983), is dubious. By the end of 25 days of hypoxia, the concentration of subunit 6 had dropped from the highest in the control period to rank fourth; no. 5 had drop
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