. Pathological technique; a practical manual for workers in pathological histology and bacteriology. ist of a generally circular expansion, with thin, trans-lucent, sharply defined margins, becoming brownish, granu-lar, and denser toward the center, which is made up of coarsebrownish clumps closely packed together (Fig. 48). PATHOGENIC BACTERIA AND FUNGI. 273 Special Culture-media.—The essential constituent ofculture-media upon which the gonococcus will grow seemsto be the blood-serum or similar albuminous fluid from theanimal body. Probably the most convenient culture-medium for thecultivatio
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. ist of a generally circular expansion, with thin, trans-lucent, sharply defined margins, becoming brownish, granu-lar, and denser toward the center, which is made up of coarsebrownish clumps closely packed together (Fig. 48). PATHOGENIC BACTERIA AND FUNGI. 273 Special Culture-media.—The essential constituent ofculture-media upon which the gonococcus will grow seemsto be the blood-serum or similar albuminous fluid from theanimal body. Probably the most convenient culture-medium for thecultivation of the gonococcus is hydrocele-fluid agar. Thismedium consists of sterile hydrocele fluid mixed with fluidagar-agar at a temperature of 400 C, in the proportion ofI part of hydrocele fluid to 2 or 3 parts of agar-agar. Thehydrocele fluid is to be obtained under the strictest precau-tions to avoid contamination with bacteria, thoroughly ster-ilized vessels, etc., being used. Ordinary tubes of plain agar-agar (2 per cent.) which havebeen previously sterilized in the usual manner are melted and. Fig. 48.— Gonococcus colony (low magnifying power; photo by L. S. Brown). brought to a temperature of 400 C. in a water-bath. To thefluid agar-agar in each tube the sterile hydrocele fluid is thenadded in the proportion of one-third to one-half the volumeof the agar-agar, care being taken to avoid the transfer of the serum to the agar-agar tubes a steril-ized pipette may be used. The tubes may then be infectedand their contents poured into sterilized Petri dishes, as inthe plate method previously described (see page 218), or thetubes may be placed on their sides in a slightly inclined 18 274 PATHOLOGICAL TECHNIQUE. position and the agar-agar allowed to solidify, thus form-ing slants which may be kept on hand ready for use. Inorder to test for the presence of contaminating bacteria inthese slants, it is well to place them in the incubator fortwenty-four hours after t
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