. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 162 C. YIN AND T. HUMPHREYS '•'. Figure I. Histoincompatihility assay. The three autograft and the three allograft combinations possible between three individual sponges are shown 72 hours after apposition of pieces of tissue on insect pins. Two apposed pieces of tissue from the same sponge remain healthy and heal together (a, b, f)- Two pieces of tissue from different sponge individuals react and create a zone of cell death exposing the skeletal matrix along the zone of allogeneic contact (c, d, e). Bar equals em. Figur
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 162 C. YIN AND T. HUMPHREYS '•'. Figure I. Histoincompatihility assay. The three autograft and the three allograft combinations possible between three individual sponges are shown 72 hours after apposition of pieces of tissue on insect pins. Two apposed pieces of tissue from the same sponge remain healthy and heal together (a, b, f)- Two pieces of tissue from different sponge individuals react and create a zone of cell death exposing the skeletal matrix along the zone of allogeneic contact (c, d, e). Bar equals em. Figure 2. Aggregates from dissociated cells from each of three individual sponges. Aggregates of pure populations of cells from the three sponges (A, B. C), and the three possible pair-wise allogeneic mixtures of dissociated cells from the three sponges (D, E. F), 2 hours after the initiation of aggregation in suspension shaker cultures. The aggregates of mixed cells from two individuals in D. E. and Fare significantly smaller than aggregates from individual pure cell aggregates in A, Band C. Bar equals mm. Figure 3. histoincompatibility reactions in aggregates containing an allogeneic mixture of dissociated cells derived from two different sponges. A. A lumpy surface at 20 hours of aggregation is the first evidence of cytotoxic reactions in mixed aggregates. B. By 21 hours the roughened appearance of the aggregate surface has become more pronounced. C. By 26 hours the mixed aggregates have begun to fall apart into smaller cell clumps which soon disintegrate completely. D. Aggregates of cells derived from one individual remain smoothly spherical as shown after 82 hours of aggregation. Bar equals Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mas
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
