Archive image from page 78 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 Sciiiiaicc-TciiiL'L'il Site Murker D12SI9S7. Digoxigenin labeling during PCR of K562 DNA with primers 1987F and 1987R was successful as shown in the approximately KB band on gel electrophoresis, an unlabeled marker band would have been at kilobases. Following hybridization of the labeled probe with a cosmid DNA dot blot, seven cosmids showed strong labeling reactions (I-IGURI'. 22). Cosmid 128D6 was a positive control for the m
Archive image from page 78 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 Sciiiiaicc-TciiiL'L'il Site Murker D12SI9S7. Digoxigenin labeling during PCR of K562 DNA with primers 1987F and 1987R was successful as shown in the approximately KB band on gel electrophoresis, an unlabeled marker band would have been at kilobases. Following hybridization of the labeled probe with a cosmid DNA dot blot, seven cosmids showed strong labeling reactions (I-IGURI'. 22). Cosmid 128D6 was a positive control for the marker and it also strongly labeled in the hybridization. Positive eosmids were as follows: c72Gl 1. cl28D6, 168C12. C192A8. cl92F12, c203F5, and c213E4. li(;iiRH22. Digoxigenin Labeled D12S1987 STS marker hybridization to cosmid dot blot. Seven cosmids showed strong labeling reactions: c72GI I, cl28D6, cl68C12, cl92A8. cl92FI2, c203F.'>, and c2l3r,4, Cosmid I28D6 was a positive control for this hybridization. Scquence-TuenedSite Marker D12S19N. An approximately kb band on agarose gel electrophoresis demonstrated the positive digoxigenin labeling of K562 DNA with primers 1914F and 1914R for STS D12S1914 during PCR. PCR of the unlabeled marker would have yielded a band length 38
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