. Advances in marine environmental research : proceedings of a symposium. Marine ecology -- Research; Marine ecology. GENERALIZED AND SIMPLIFIED PROTOCOL FOR POINT MUTATION IN CELLULAR SYSTEMS CELL INOCULATION AND EXPOSURE TO TEST AGENT MUTANT EXPRESSION MUTANT CELL SELECTION MUTANT CELL ANALYSIS ATTACHMENT WASH Figure 7-2. A generalized and simplified protocol for the induction, isolation and characterization of mutations in cellular systems. Figure 7-3 represents a protocol containing the same basic features as that in Figure 7-2, but is specific for the CHO Cell BrdU-VL system. In this tech
. Advances in marine environmental research : proceedings of a symposium. Marine ecology -- Research; Marine ecology. GENERALIZED AND SIMPLIFIED PROTOCOL FOR POINT MUTATION IN CELLULAR SYSTEMS CELL INOCULATION AND EXPOSURE TO TEST AGENT MUTANT EXPRESSION MUTANT CELL SELECTION MUTANT CELL ANALYSIS ATTACHMENT WASH Figure 7-2. A generalized and simplified protocol for the induction, isolation and characterization of mutations in cellular systems. Figure 7-3 represents a protocol containing the same basic features as that in Figure 7-2, but is specific for the CHO Cell BrdU-VL system. In this technique, CHO-Kl cells are cultured in two types of media. One medium (F12D) contains the minimal nutritional requirements for the growth of single cells into macroscopic colonies with high efficiency. A second, enriched medium (F12) is constructed from the minimal by addition of nine nutrilites (alanine, glycine, aspartic acid, glutamic acid, lipoic acid, vitamin Bj2, inositol, A B C chemical treatment mutation induction. mutant cell selection ?> 5-bromodeoxyuridine. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Environmental Research Laboratory (Narragansett, R. I. ); Jacoff, Francine Sakin. Narragansett, R. I. : Environmental Research Laboratory, Office of Research and Development, U. S. Environmental Protection Agency
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