Electrolytes in biological systems, incorporating Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 electrolytesinbi00shan Year: 1955 DEAN B. COWIE AND RICHARD B. ROBERTS 11 T. utilis on the other hand removed 76%%%%%%%%%%%%%%%% of the original radioactivity. From this loss of radioactivity from the immersion fluid it can be calculated that the water space would exceed the total cell volume by more than a factor of 3. This absurd result clearly indicates that the supernatant
Electrolytes in biological systems, incorporating Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 electrolytesinbi00shan Year: 1955 DEAN B. COWIE AND RICHARD B. ROBERTS 11 T. utilis on the other hand removed 76%%%%%%%%%%%%%%%% of the original radioactivity. From this loss of radioactivity from the immersion fluid it can be calculated that the water space would exceed the total cell volume by more than a factor of 3. This absurd result clearly indicates that the supernatant analysis method, though Fig. 4. Radiosulfate uptake by 7\ iililis. Curve \ represents radioactivity of TCA pre- cipitable fraction observed during the growth of the cells shown in Curve B. The ratio A/B shows the direct correlation between growth and sulfur uptake of the TCA precipitable frac- tion (proteins). satisfactory for studies of E. coli permeability, is not necessarily adequate when other organisms are investigated. For T. utilis it is also necessary to measure the radioactivity retained by the cells through metabolic incorpora- tion. Table 6 shows that even when the quantity of sulfur in the medium was low only a slight amount of radioactivity was retained by E. coli. In contrast, T. utilis retained 61 %%%%%%%%%%%%%%%% of the total sulfur originally available. When this meta-
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