. Applied bacteriology for nurses. e of varyingpower and are usually numbered from 1 to 5. Attachedto the lower end of the tube is a so-called nose-piece,carrying two or three objectives, each objective con-sisting of a series of lenses mounted together. Theobjectives are usually distinguished by numbers (3, 5,and 7) or by fractions (§, J, and J inch). Whenusing the higher powers of the microscope it is importantto have strong illumination from the mirror. In orderto bring this about a series of lenses, called the con-denser, is placed between the mirror and the even with this equipm
. Applied bacteriology for nurses. e of varyingpower and are usually numbered from 1 to 5. Attachedto the lower end of the tube is a so-called nose-piece,carrying two or three objectives, each objective con-sisting of a series of lenses mounted together. Theobjectives are usually distinguished by numbers (3, 5,and 7) or by fractions (§, J, and J inch). Whenusing the higher powers of the microscope it is importantto have strong illumination from the mirror. In orderto bring this about a series of lenses, called the con-denser, is placed between the mirror and the even with this equipment it is difficult to studythe finer details of bacterial structure. This can only 22 METHODS OF STUDYING BACTERIA 23 be satisfactorily accomplished by staining the bacteriaand examining them by means of very high-power ob-jectives, which dip into a drop of cedar oil placeddirectly on the specimen to be examined. Such objec-tives are spoken of as oil-immersion objectives; the one OraduatedDraw-tube, Coarse Adjuster Pine Adjuster. Objectives andNose-piece Stage Siihstarje withDin])hrngmand Condenser Fig. 7.—Bactcriologic microscope (Ball). in common use, the ^^ inch, when used with a No. 5eye-piece, magnifies about 1200 times. For the stainingof bacteria we usually employ some of the coal-tar dyes^such as methylene-bluc, fuchsin, gentian-violet, appearance of such stained bacteria is well shownin Plate 1. The use of these stains has a further 24 APPLIED BACTERIOLOGY FOR NURSES value, in that it often helps to differentiate bacteriafrom one another. This will be seen when we studyGrams stain and the stain for tubercle bacilh (p. 26). Demonstration.—Show a reading glass, and a high-power magni-f>dng glass such as is used to count threads in a woven fabric.(Image erect.) Demonstrate the compound microscope. (Image inverted.) CHAPTER IVPREPARATION OF STAINED SMEARS In preparing bacteria for microscopic examination atiny bit of the material (pus^ sputum, exudate, cul
Size: 1207px × 2070px
Photo credit: © The Reading Room / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1919
