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. dan bai zhi zu xue cong xu lie dao gong neng. botany. 第äºç« åºå 表达çæ£æµæ¹æ³ 31 Poly A + RNA çç©ç´ æ è®°ç o/igo dT. (6) Main '1 éå¶æ§ISåæ¶åï¼çº¯å åæ ç-¾å¹¶è¿æ¥è¿æ¥å- (7) J1 å éå¹¶æ¸¬åº G4r(XCTAGTCAGGCGACTTCAC/47(^CAAAGTGCTTTCGAGGAr-47(7GAAGTCCTACGATCATGGC/17"G æ ç-¾3 æ ç-¾4 æ ç-¾5 æ ç-¾6 V åæ ç-¾ åæ ç-¾S åæ ç-¾C å¾åºå 表达系ååæï¼SAGE)æ¹æ³ç¤ºæå¾ æ-¥éª¤1:ç¨çç©ç´ -oligodTå¼ç©å°PolyA+çRNA转æ¢ä¸ºcDNAãæ-¥éª¤2:åæçcDNAç¨é«é¢ åå²çéå¶æ§æ ¸é ¸å åé µï¼"éå®é ¶"ï¼ï¼å¦iV/a in. 4bpè¯å«ä½ç¹ï¼å¹³å256æ ¸æé ¸ä¸ä¸ªé ¶åä½ ç¹ï¼æ¶åãæ-¥éª¤3: cDNAç3'端被çç©ç´ å 裹çç£ç æè·ãcDNAæ± è¢«ä¸åä¸ºäº ï¼ "Aæ± "å "Bæ± "ï¼ï¼ä¸åçæ¥å¤´è¢«è¿æ¥å°æ¯ä¸ªcDNAæ± ä¸-ãæ-¥éª¤4:è¿äºæ¥å¤´å«ælisåéå¶æ§æ ¸é ¸å å »ä½ç¹ï¼tagging enzyme (å¦æ-¤å½åæ¯å 为å®ä»¬çåå²ä½ç¹ç¦»è¯å«ä½ç¹æç¡®å®ç碱åºæ°ï¼æ¯å¦ Fok Iå¨å®çè¯å«ä½ç¹ä¸æ¸¸13个碱åºå¤åå²ï¼è¢«ç¨æ¥åå²cDNA/æ¥å¤´æåå-ï¼éæ¾åºéçå¨ æ¥å¤´ä¸çcDNAç-åºåæ ç-¾ãæ-¥éª¤5:æ¥å¤´-æ ç-¾æååå-尾对尾ç¸è¿ï¼PCRæ©å¢ï¼éè¿æ¥å¤´ åºåï¼ãæ-¥éª¤6:ç¨éå®é µå°æ¥å¤´ä»åæ ç-¾ä¸å»é¤ï¼çº¯ååæ ç-¾å¹¶å°å®ä»¬å½¼æ-¤è¿æ¥å½¢æè¿æ¥å- (concatemer)ãæ-¥éª¤7:è¿æ¥å-å éä¸æµåºï¼åç°æ¯ä¸ªæ ç-¾çç¹å¾ã. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original S. R. Pennington M. J. Dun
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