. Carnegie Institution of Washington publication. WILT-DISEASES OF TOBACCO. 243 Nicotiana rustica is not attacked, and the following varieties of N. tabacum are not much subject: Ohasama, Taketadate, Mitsuke, Kentucky White, Green River Prior. Inoculations succeeded on Physalis minimum, Capsicum longum, Amaranthus gangeticus, and Polygonum tinctorum; they failed on Solatium mclongena, Lycopersicum esculcntum, and Physalis alkekengi. No details are given as to methods of inoculation, phenomena produced, or number of experiments. Nitrogenous fertilizers predispose to disease; pot- ash salts do n
. Carnegie Institution of Washington publication. WILT-DISEASES OF TOBACCO. 243 Nicotiana rustica is not attacked, and the following varieties of N. tabacum are not much subject: Ohasama, Taketadate, Mitsuke, Kentucky White, Green River Prior. Inoculations succeeded on Physalis minimum, Capsicum longum, Amaranthus gangeticus, and Polygonum tinctorum; they failed on Solatium mclongena, Lycopersicum esculcntum, and Physalis alkekengi. No details are given as to methods of inoculation, phenomena produced, or number of experiments. Nitrogenous fertilizers predispose to disease; pot- ash salts do not. Early planting is the best protec- tion. Through the courtesy of Dr. Uyeda, who sent alcoholic material, I was enabled to study his dis- ease in serial sections. The bacteria closely resemble Bad. solanacea- rum in general appearance. They are enormously abun- dant in vessels of the wood, in inner and outer phloem, in the cortical parenchyma, andinthepith. I also found them to have made cavities Fig. 131.* resembling those due to Bad. solanacearum. They stained deep red with carbol fuchsin and were quite uniform in appearance. Spores were not observed. (Compare figs. 131 and 132 with those made from stems of the North Carolina to- bacco, figs. 116 and 117.) Also, with bacteria taken from this Japanese tobacco stem I made several attempts to stain theflagella. At first I got no indications of peritrichiate rods and many faint indications of Fig Fig. 1334 polar fiagella; nevertheless no well-stained ones, such as could be relied on for demonstration. *Fig. 131.—Cross-section of a tobacco-stem received from Uyeda, showing character of the bacterial occupation in the Japanese tobacco wilt (compare with fig. 117). The section is taken from the wood midway between pith and bark, the cambium being in the direction of the arrow. The vessels next beyond 1', 1", 1*. I", Fare also occupied by the bacteria, as well as many others. Middle section of slide 482, stained
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