. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 364 P. WADSWORTH AND R. D. SLOBODA => k. o -2 2 W _L I J- - i i 450 500 wavelength (nm) 550 FIGURE 5. Excitation/emission spectra of DTAF labeled microtubule proteins. The excitation maximum (—x—X—) is 495 nm and the emission maximum (—•—•—) is 520 nm. These values are the same whether intact MTs or depolymerized MT proteins are used for the determination. AU = arbitrary units. * * I. b FIGURE 6. Polarized light micrographs demonstrating the effect of fluorescent microtubule proteins on the birefringence of isolated meiot
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 364 P. WADSWORTH AND R. D. SLOBODA => k. o -2 2 W _L I J- - i i 450 500 wavelength (nm) 550 FIGURE 5. Excitation/emission spectra of DTAF labeled microtubule proteins. The excitation maximum (—x—X—) is 495 nm and the emission maximum (—•—•—) is 520 nm. These values are the same whether intact MTs or depolymerized MT proteins are used for the determination. AU = arbitrary units. * * I. b FIGURE 6. Polarized light micrographs demonstrating the effect of fluorescent microtubule proteins on the birefringence of isolated meiotic spindles from the surf clam Spisula solidissima. (a) Polarized light micrograph of the spindles immediately after isolation, (b) The same preparation of spindles after centrifugation and resuspension in PM buffer at 4°C. When resuspended in PM buffer containing (c) mg/ml and (d) mg/ml fluorescent microtubule proteins, followed by incubation at 37°C, BR is observed in proportion to the concent-ation of fluorescent proteins Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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