. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTEIN ACTIVATION OF ARBACIA SPERM 425 80 r- 60- c M f 40 o> O_ 20 Protein _n_ • — cotolose 2 D — cryst BSA 2 A — Cohn froct ¥ 2 O -SOD 2 A - SBTI 1 • — gelatin 1. 5 17 50 Protein concentration: ^.o/ml 167 500 FIGURE 2. Reactivation by proteins of fertilizing capacity of aged Arbacia sperm as a function of protein concentration (^g/ml). Procedures for inactivation and for determination of fertilizing capacity are given in Materials and Methods. Protein concentrations on the abscissa are plotted in a logarithmic scale
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTEIN ACTIVATION OF ARBACIA SPERM 425 80 r- 60- c M f 40 o> O_ 20 Protein _n_ • — cotolose 2 D — cryst BSA 2 A — Cohn froct ¥ 2 O -SOD 2 A - SBTI 1 • — gelatin 1. 5 17 50 Protein concentration: ^.o/ml 167 500 FIGURE 2. Reactivation by proteins of fertilizing capacity of aged Arbacia sperm as a function of protein concentration (^g/ml). Procedures for inactivation and for determination of fertilizing capacity are given in Materials and Methods. Protein concentrations on the abscissa are plotted in a logarithmic scale. The inset shows the symbols for the proteins and n, the numbers of experiments for each. experiments was great is indicated by the standard errors. In these three experiments, however, in no instance was the level of polyspermy induced by a protein in a suspension of sperm and eggs less than 2-fold greater than its control. Figure 4 shows levels of polyspermy induced by the six proteins (all at 2 mg/ml) in a series of experiments (not every protein was tested in each experiment). Again, 200 o 9) o> Q o o FIGURE 3. Motility of aged Arbacia sperm reactivated with proteins, presented relative to the motility of aged sperm, and determined as described in Materials and Methods. The motility of aged sperm not treated with proteins, set at 100, was approximately 25% of the motility of fresh sperm. Numbers of experiments are shown; the error bars show SEMs for BSA, SBTI, and CAT (n > 3), and total ranges for SOD and CFV (n = 2).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 189
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
