. Cytology. Cytology. CYTOPLASM PROTEIN 1 i k s ^ ^ N ^>> .^ 2400 2600 2800 3000 520O A WAVE LENOTH FAR "SAP" MOTCIN & o z X V V. â ^ ^^l\ 1 v 2'00 26M 2W0 9000 S200A WAVE LENOTH NUCLEOLUS \. N L RNA ,^ â¢Â»â PdOTEIM 1 V s. i ^ X V \^ V 24 OO 2600 CHROMATI 2600 3000 3200 A WAVE LENGTH ! A DNA + PROTEIN vV i \ i \ X w » \ , â ^ - M 100 » 100 21 too 9 w« 900 /E 1 3( .EN( iOOA ITH Figure 11-18. Ultraviolet Absorption Spectra of Various Cell Com- ponents as Measured by Ultraviolet Microspectrophotometry. (From Giese, A. C, 1957. "Cell Physiology," W. B. Saun
. Cytology. Cytology. CYTOPLASM PROTEIN 1 i k s ^ ^ N ^>> .^ 2400 2600 2800 3000 520O A WAVE LENOTH FAR "SAP" MOTCIN & o z X V V. â ^ ^^l\ 1 v 2'00 26M 2W0 9000 S200A WAVE LENOTH NUCLEOLUS \. N L RNA ,^ â¢Â»â PdOTEIM 1 V s. i ^ X V \^ V 24 OO 2600 CHROMATI 2600 3000 3200 A WAVE LENGTH ! A DNA + PROTEIN vV i \ i \ X w » \ , â ^ - M 100 » 100 21 too 9 w« 900 /E 1 3( .EN( iOOA ITH Figure 11-18. Ultraviolet Absorption Spectra of Various Cell Com- ponents as Measured by Ultraviolet Microspectrophotometry. (From Giese, A. C, 1957. "Cell Physiology," W. B. Saunders Co., Philadelphia, Pa., Fig. , p. 167. After Caspersson, T. O., 1950. "Cell Growth and Cell Func- tion," W. W. Norton Co., Inc., New York, N. Y., Fig. 32, p. 79.) the ultraviolet spectra of many organic compounds frequently show only one or two absorption peaks, it is usually not possible to identify a substance entirely by its ultraviolet absorption (Figure 11-19). With the possible exception of tryptophan and tyrosine, which absorb maxi- mally at"280 m//, most proteins of the cell absorb at wavelengths shorter than 230 mn. The high specific absorption of nucleic acids in the 238 / CHAPTER 11. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Wilson, G. B. (George Bernard), 1914-; Morrison, John H. (John Herbert), 1927-. New York, Reinhold
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