. Adventures in radioisotope research;. Radioactive tracers; Radiobiology. RADIOACTIVE INDICATORS IX BIOCHEMISTRY 991 NyLiN(53) made a very extended application of the method described. He determined, besides the total circulating erythrocyte volume, that of single organs such as lungs and legs. A blood sample of a human subject was secured and labelled with 32p ^s described above. Before injection of an aliquot of the labelled red corpuscles into the subject, the vessels were clamped. The red corpuscles circulating in the legs were thus prevented from participating in the "dilution"
. Adventures in radioisotope research;. Radioactive tracers; Radiobiology. RADIOACTIVE INDICATORS IX BIOCHEMISTRY 991 NyLiN(53) made a very extended application of the method described. He determined, besides the total circulating erythrocyte volume, that of single organs such as lungs and legs. A blood sample of a human subject was secured and labelled with 32p ^s described above. Before injection of an aliquot of the labelled red corpuscles into the subject, the vessels were clamped. The red corpuscles circulating in the legs were thus prevented from participating in the "dilution" of the injected labelled erythrocytes, which takes place after injection of labelled red. 400 360 360 Circulation fo the legS released Red blood corpuscles Plasma Circulat. bl. volume leiOgr 2790" 4600" 27gr/kg 42 « 69 II 1645 ml 2730 " 4405" 25 ml/kg 41 " 66 10 20 30 40 Minutes after injection 50 60 Fig. 13. — Determination of the circulating corpuscle (blood) volume of the legs (Nylin) (reproduced, with permission, from Ark. Kemi 20, A No. 17 (1945). corpuscles into the non-radioactive circulation and the extent of which indicates the amount of circulating erythrocytes. As seen in Fig. 13, 10 minutes after injection of labelled corpuscles, the amount of circulating erythrocytes, excluding that of the legs, is found from the "dilution" figures to be 1560 gm. After removal of the clamps, there was a marked decrease in the activity of a 1 gm sample of corpuscle (denoted in Fig. 13 as specific activity). Owing to the participation of the red corpuscles of the leg in the "dilution" process, the specific activity of the corpuscles decreases from 550 to 428, indicating that the red corpuscle volume of the body, which now includes that of the legs as well, amounts to 1810 gm From the above figures the weight of the erythrocytes circulating in the legs is determined to be 250 gm. The labelling of a red corpuscle sample in vitro is made
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