. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 36 ?30 0 -24 g« IT Q. |2 CO o 6. 42 X O cr LJ 20 40 80 IOO 120 TUBE NUMBER FIGURE 6. Elution patterns from DEAE cellulose column. The pH curve is designated by: A-A, Cl (meq/1.) by Q-O ; and prcjtein by •-•, shaded area = to the mixing chamber contained 700 ml. of the same buffer at pTI After the above elution was complete, the mixing chamber was charged with 500 ml. Tris: maleate M, pH , and 200 ml. A' XaCl was added to the deliver) flask. \Yhen 100 ml. ± 25 ml. remained in the mixing chamber, 2
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 36 ?30 0 -24 g« IT Q. |2 CO o 6. 42 X O cr LJ 20 40 80 IOO 120 TUBE NUMBER FIGURE 6. Elution patterns from DEAE cellulose column. The pH curve is designated by: A-A, Cl (meq/1.) by Q-O ; and prcjtein by •-•, shaded area = to the mixing chamber contained 700 ml. of the same buffer at pTI After the above elution was complete, the mixing chamber was charged with 500 ml. Tris: maleate M, pH , and 200 ml. A' XaCl was added to the deliver) flask. \Yhen 100 ml. ± 25 ml. remained in the mixing chamber, 200 ml. of A' NaCl were added to the delivery flask and elution continued. Fifteen-milliliter- samples were collected throughout the elution. The profile of protein elutecl from the column is shown in Figure 6. The pH gradient is presented, as well as the Cl' concentration as meq/1. Chloride was determined with a Cotlove chloridometer and pH with a Beckman probe-type electrode. Beta-glucosidase activity is indicated by the hatched area. Beta-glucosidase activity and a trace of /3-galactosidase. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
