. Annual report of the New York State College of Agriculture at Cornell University and the Agricultural Experiment Station. New York State College of Agriculture; Cornell University. Agricultural Experiment Station; Agriculture -- New York (State). 172 Bulletin 395 take the place of the phloem and the inner cortex. In cross section this group of cells is wedge-shaped, with the apex extending toward the center of the cane, and in the outer edge small bundles of fibers frequently may- be observed. This tissue collapses soon after development and is torn apart when the fissures occur in the lesio
. Annual report of the New York State College of Agriculture at Cornell University and the Agricultural Experiment Station. New York State College of Agriculture; Cornell University. Agricultural Experiment Station; Agriculture -- New York (State). 172 Bulletin 395 take the place of the phloem and the inner cortex. In cross section this group of cells is wedge-shaped, with the apex extending toward the center of the cane, and in the outer edge small bundles of fibers frequently may- be observed. This tissue collapses soon after development and is torn apart when the fissures occur in the lesion. CULTURAL CHARACTERS Miss Stoneman (1898) states that this fungus "does not adapt itself readily to artificial culture, and considerable difficulty was experienced before obtaining a pure ; In making plantings of diseased tissue the writer found that the fungus develops so slowly that contaminations are likely to grow over it. The growth of the or- ganism from such plantings cannot be detected mi- croscopically for five or six days, and a week more is required for sufficient develop- ment before the fungus can be re- moved to a test tube. Spore dilu- tion plates are as satisfactory a method of isolat- ing the pathogene as any, and conidia are readily obtainable throughout the summer and even late in the autumn. A pure culture of the fungus may be obtained also by inverting a sterilized petri dish containing a thin layer of nutrient agar over a small piece of raspberry cane bearing ascocarps. When the piece of bark is moistened the ascospores are ejected from the asci and lodge on the agar surface above. The spores can then be located easily with a low-magnifying microscope. By marking the position of the spores on the lower side of the petri dish their germination and development can be observed. The ascospores first produce sprout conidia, but further development on the medium is identical with that from the conidia of the fungus. 282. Fig. 18. CONIDIA of p
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