. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. OSMOTIC PROPERTIES OF THE ERYTHROCYTE 109 Since the cell is impermeable to hemoglobin and to base but perme- able to water, (Hb)c and (B)c will remain constant, while [Hb]c and [B]c will tend to vary. Indeed, the values of the latter quantities may be used as a measure of the volume of the " liquid " portion of the cell and indirectly of that of the cell as a whole, provided that information is available concerning the bulk of the " non-liquid " materials. The erythrocyte is known to be permeable to the o
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. OSMOTIC PROPERTIES OF THE ERYTHROCYTE 109 Since the cell is impermeable to hemoglobin and to base but perme- able to water, (Hb)c and (B)c will remain constant, while [Hb]c and [B]c will tend to vary. Indeed, the values of the latter quantities may be used as a measure of the volume of the " liquid " portion of the cell and indirectly of that of the cell as a whole, provided that information is available concerning the bulk of the " non-liquid " materials. The erythrocyte is known to be permeable to the osmotically important anions, so neither (A}c nor [A]c will be fixed but will in general vary in such a way that the Donnan ratio, [/i]c/[^].s, will have the value determined by the other properties of the system. (The use of con- centrations in place of activities introduces no great error; for the justi- fication for assuming complete dissociation of the various salts involved -Van Slyke, Wu and McLean (1923) — may be consulted.) If now in equation (2), (J5)c-- (BHb)c be substituted for (A)c, 2(A)S be substituted for (B}s-\- (A)8 and the terms be suitably re- arraned we obtain : 2(A} CH20)C~"2(5)C- -(BHb)c + (Hb)c' Similarly, by substituting in equation (2) (A}c-\- (BHb)c for 2(A)S for (B)s-\- (A)s, and rearranging, we have: _ — v •<i ; f i / j \ 7TTO)7r : (H20), (H20), Dividing both sides of equation (4) by 2(A)S/(H2O)S, substituting the value of (H2O)g/(H2O)c from equation (3) and remembering the definitions of [A]c and [A]s and of the Donnan ratio, r, we have finally: cr -0 + (Hb)/ Up to this point Van Slyke, Wu and McLean have been followed in principle, and equation (5) is the same as their equation (14) except that (BP}S, the base bound by the protein in the solution, is here zero, there being no protein present in the solution. The base bound by protein in the cell is also here designated as (BHb)c instead of as (£P),.. Beyond this point a somewhat differ
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