. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. 268 Phylum Arthropoda Order odonata CULTURE METHODS FOR THE DAMSELFLY, ISCHNURA VERTICALIS Evelyn George Grieve, Cornell University REARING work was begun with the full-grown nymphs which were . collected early in the spring from a small, grassy fish pond. From the first generation, which were the stock adults, eggs were obtained for life history studies. As soon as the nymphs hatched they were isolated and reared in separate containers for the entire life spa


. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. 268 Phylum Arthropoda Order odonata CULTURE METHODS FOR THE DAMSELFLY, ISCHNURA VERTICALIS Evelyn George Grieve, Cornell University REARING work was begun with the full-grown nymphs which were . collected early in the spring from a small, grassy fish pond. From the first generation, which were the stock adults, eggs were obtained for life history studies. As soon as the nymphs hatched they were isolated and reared in separate containers for the entire life span. ..,...,,,,JiV,n Cages. The type of cage ..:â â¢-* "'. '~> ""'""N" ; If**j (Fig. 61) used for breeding ^f->^M':: .$£':/-'M stock was a fairly large aquarium covered with a screen cage, fitting the aquarium closely at the sides, and allowing room above for the adults to fly, mate, and capture their prey. Aquatic plants, prin- cipally Eleocharis palustris, were kept growing in one corner of the aquarium; up these the nymphs could crawl to transform. When the females were ready to oviposit, one or two of the flexible stems of E. palustris were bent down into the water where they were held by the surface film. The females preferred to oviposit in these floating stems, and each day eggs could be removed and dated for the study of embryonic development. In order to keep records of all individuals, and also because the species is cannibalistic, separate containers were used for all reared specimens. The most satisfactory type for the young nymphs was a small boat (Fig. 62), floating with others in a large pan of water. The frame of the boat is of balsa wood, which is very light and buoyant, and the "hold" of the vessel is made of silk. Although the illustration of the boat is about natural size, the silk was of finer mesh than that shown, namely about. Breeding cage for Ischnura Please note that these images are e


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